• Embargo End Date: 2025-05-14

Date: 2020-01-01

Creator: Emma Victoria Bertke

Access: Embargoed

Date: 2024-01-01

Creator: Katrina Carrier

Access: Open access

The ability of nervous systems to maintain function when exposed to global perturbations in temperature and salinity is a non-trivial task. The nervous system of the American lobster (H. americanus), a marine osmoconformer and poikilotherm, must be robust to these stressors, as they frequently experience fluctuations in both. I characterized the effects of temperature on the output of the pyloric circuit, a central pattern generator in the stomatogastric nervous system (STNS) that controls food filtration and established the maximum temperature that neurons in this circuit can withstand without “crashing” (ceasing to function but recovering when returned to normal conditions). I established a range of saline concentrations that did not cause the system to crash, and then determined whether combinatorial changes in temperature and salinity concentrations alter the maximum temperature the system tolerated. Even as burst frequency increased as temperature increased, phase constancy was observed. Interestingly, the system crashed at higher temperatures upon exposure to lower saline concentrations and lower temperatures in higher saline concentrations. I also established the range of saline concentrations that the lobster’s whole heart and cardiac ganglion (CG), the nervous system that controls the lobster’s heartbeat, can withstand. Then, I examined whether exposure to altered salinity and elevated temperature alters the crash temperature of the whole heart and CG. The CG crashed at higher temperatures than the whole heart in each saline concentration. Like the STNS, the whole heart and CG both crashed at higher temperatures in lower saline concentrations and higher temperatures in lower saline concentrations.

• Restriction End Date: 2026-06-01

Date: 2023-01-01

Creator: Eliza M. Rhee

Access: Access restricted to the Bowdoin Community

Date: 2014-05-01

Creator: Elizabeth A Owens

Access: Open access

The American lobster, Homarus americanus, inhabits a large oceanic range spanning from Labrador, Canada to North Carolina, USA. This geographic range varies in temperature by as much as 25ºC, and daily temperature fluctuations of up to 12ºC may occur at a single location depending on season, water depth, and tides. The cardiac system of the lobster is sensitive to these temperature changes, and has been shown to adjust its functioning over a large temperature range. A previous study showed that various functional parameters respond differently to temperature changes, but a stable cardiac output can be maintained over the range of 2-20ºC. The current study showed that the effects of temperature were exerted primarily through changes in the lobster heart central pattern generator, the cardiac ganglion. Similar patterns of change were seen in both semi-intact hearts and isolated cardiac ganglion preparations in response to increasing temperature. Specifically, with increasing temperature, the burst frequency showed a biphasic pattern in which frequency initially increased, then decreased rapidly at high temperatures. The burst duration, duty cycle, and number of spikes per burst generally decreased with increasing temperature, and spike frequency increased over the entire temperature range. Semi-intact hearts and isolated cardiac ganglia showed similar “crash” patterns, characterized by complete loss of function at high temperatures and complete recovery of function when temperature was returned to baseline. Feedback in the semi-intact heart provided some stabilization of bursting activity, but it did not provide the expected protection from high temperatures. The isolated CG had a significantly higher crash temperature than did the semi-intact system. This discrepancy in crash temperatures may be explained by considering factors at the level of the muscle and neuromuscular junction (NMJ), such as stretch and nitric oxide (NO) feedback and the balance of facilitation and depression at the NMJ. Stimulated preparations showed defacilitation of contraction amplitude at high temperatures despite the maintenance of constant burst parameters of stimulation. Therefore, several factors contributing to the relatively low crash temperature of the intact system may be a shift in the balance of facilitation and depression at the NMJ, a depression in ganglion function due to the release of NO by the muscle, or a combination of the two mechanisms.

Date: 2020-01-01

Creator: Marie Marjorie Bergsund

Access: Access restricted to the Bowdoin Community