Showing 2011 - 2020 of 5831 Items
Non-genomic effects of steroids on teleost fish olfaction: behavioral and anatomical approaches Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2020-01-01
Creator: Leah B Kratochvil
Access: Access restricted to the Bowdoin Community
Identification of the determinants for the specific recognition of single-strand telomeric DNA by Cdc13
Date: 2006-01-24
Creator: Aimee M. Eldridge, Wayne A. Halsey, Deborah S. Wuttke
Access: Open access
- The single-strand overhang present at telomeres plays a critical role in mediating both the capping and telomerase regulation functions of telomeres. The telomere end-binding proteins, Cdc13 in Saccharomyces cerevisiae, Pot1 in higher eukaryotes, and TEBP in the ciliated protozoan Oxytricha nova, exhibit sequence-specific binding to their respective single-strand overhangs. S. cerevisiae telomeres are composed of a heterogeneous mixture of GT-rich telomeric sequence, unlike in higher eukaryotes which have a simple repeat that is maintained with high fidelity. In yeast, the telomeric overhang is recognized by the essential protein Cdc13, which coordinates end-capping and telomerase activities at the telomere. The Cdc13 DNA-binding domain (Cdc13-DBD) binds these telomere sequences with high affinity (3 pM) and sequence specificity. To better understand the basis for this remarkable recognition, we have investigated the binding of the Cdc13-DBD to a series of altered DNA substrates. Although an 11-mer of GT-rich sequence is required for full binding affinity, only three of these 11 bases are recognized with high specificity. This specificity differs from that observed in the other known telomere end-binding proteins, but is well suited to the specific role of Cdc13 at yeast telomeres. These studies expand our understanding of telomere recognition by the Cdc13-DBD and of the unique molecular recognition properties of ssDNA binding. © 2006 American Chemical Society.
A record of atmospheric halocarbons during the twentieth century from polar firn air
Date: 1999-06-24
Creator: James H. Butler, Mark Battle, Michael L. Bender, Stephen A. Montzka, Andrew D., Clarke, Eric S. Saltzman, Cara M. Sucher, Jeffrey P. Severinghaus, James W. Elkins
Access: Open access
- Measurements of trace gases in air trapped in polar firn (unconsolidated snow) demonstrate that natural sources of chlorofluorocarbons, halons, persistent chlorocarbon solvents and sulphur hexafluoride to the atmosphere are minimal or non-existent. Atmospheric concentrations of these gases, reconstructed back to the late nineteenth century, are consistent with atmospheric histories derived from anthropogenic emission rates and known atmospheric lifetimes. The measurements confirm the predominance of human activity in the atmospheric budget of organic chlorine, and allow the estimation of atmospheric histories of halogenated gases of combined anthropogenic and natural origin. The pre-twentieth-century burden of methyl chloride was close to that at present, while the burden of methyl bromide was probably over half of today's value.
Disruption of topoisomerase II perturbs pairing in Drosophila cell culture
Date: 2007-09-01
Creator: Benjamin R. Williams, Jack R. Bateman, Natasha D. Novikov, C. Ting Wu
Access: Open access
- Homolog pairing refers to the alignment and physical apposition of homologous chromosomal segments. Although commonly observed during meiosis, homolog pairing also occurs in nonmeiotic cells of several organisms, including humans and Drosophila. The mechanism underlying nonmeiotic pairing, however, remains largely unknown. Here, we explore the use of established Drosophila cell lines for the analysis of pairing in somatic cells. Using fluorescent in situ hybridization (FISH), we assayed pairing at nine regions scattered throughout the genome of Kc167 cells, observing high levels of homolog pairing at all six euchromatic regions assayed and variably lower levels in regions in or near centromeric heterochromatin. We have also observed extensive pairing in six additional cell lines representing different tissues of origin, different ploidies, and two different species, demonstrating homolog pairing in cell culture to be impervious to cell type or culture history. Furthermore, by sorting Kc167 cells into G1, S, and G2 subpopulations, we show that even progression through these stages of the cell cycle does not significantly change pairing levels. Finally, our data indicate that disrupting Drosophila topoisomerase II (Top2) gene function with RNAi and chemical inhibitors perturbs homolog pairing, suggesting Top2 to be a gene important for pairing. Copyright © 2007 by the Genetics Society of America.
A 350-year atmospheric history for carbonyl sulfide inferred from Antarctic firn air and air trapped in ice
Date: 2004-11-27
Creator: Stephen A. Montzka, M. Aydin, M. Battle, J. H. Butler, E. S., Saltzman, B. D. Hall, A. D. Clarke, D. Mondeel, J. W. Elkins
Access: Open access
- Carbonyl sulfide (COS) and other trace gases were measured in firn air collected near South Pole (89.98°S) and from air trapped in ice at Siple Dome, Antarctica (81.65°S). The results, when considered with ambient air data and previous ice core measurements, provide further evidence that atmospheric mixing ratios of COS over Antarctica between 1650 and 1850 A.D. were substantially lower than those observed today. Specifically, the results suggest annual mean COS mixing ratios between 300 and 400 pmol mol-1 (ppt) during 1650-1850 A.D. and increases throughout most of the twentieth century. Measurements of COS in modern air and in the upper layers of the firn at South Pole indicate ambient, annual mean mixing ratios between 480 and 490 ppt with substantial seasonal variations. Peak mixing ratios are observed during austral summer in ambient air at South Pole and Cape Grim, Tasmania (40.41°S). Provided COS is not produced or destroyed in firn, these results also suggest that atmospheric COS mixing ratios have decreased 60-90 ppt (10-16%) since the 1980s in high latitudes of the Southern Hemisphere. The history derived for atmospheric mixing ratios of COS in the Southern Hemisphere since 1850 is closely related to historical anthropogenic sulfur emissions. The fraction of anthropogenic sulfur emissions released as COS (directly or indirectly) needed to explain the secular changes in atmospheric COS over this period is 0.3-0.6%. Copyright 2004 by the American Geophysical Union.
Wnt Signaling is Dispensable to Formation of the First Tooth in D. Rerio This record is embargoed.
- Embargo End Date: 2025-05-14
Date: 2020-01-01
Creator: Zachary C. LeBlanc
Access: Embargoed
Bowdoin College Catalogue (1922-1923)
Date: 1923-01-01
Access: Open access
- Bowdoin College Bulletin no. 122
One-loop SYM-supergravity relation for five-point amplitudes
Date: 2011-11-21
Creator: Stephen G. Naculich, Howard J. Schnitzer
Access: Open access
- We derive a linear relation between the one-loop five-point amplitude of N = 8 supergravity and the one-loop five-point subleading-color amplitudes of N = 4 supersymmetric Yang-Mills theory. © 2011 SISSA.