Showing 2021 - 2030 of 5831 Items
Haplotype mapping of a diploid non-meiotic organism using existing and induced aneuploidies
Date: 2008-01-01
Creator: Melanie Legrand, Anja Forche, Anna Selmecki, Christine Chan, David T., Kirkpatrick, Judith Berman
Access: Open access
- Haplotype maps (HapMaps) reveal underlying sequence variation and facilitate the study of recombination and genetic diversity. In general, HapMaps are produced by analysis of Single-Nucleotide Polymorphism (SNP) segregation in large numbers of meiotic progeny. Candida albicans, the most common human fungal pathogen, is an obligate diploid that does not appear to undergo meiosis. Thus, standard methods for haplotype mapping cannot be used. We exploited naturally occurring aneuploid strains to determine the haplotypes of the eight chromosome pairs in the C. albicans laboratory strain SC5314 and in a clinical isolate. Comparison of the maps revealed that the clinical strain had undergone a significant amount of genome rearrangement, consisting primarily of crossover or gene conversion recombination events. SNP map haplotyping revealed that insertion and activation of the UAU1 cassette in essential and non-essential genes can result in whole chromosome aneuploidy. UAU1 is often used to construct homozygous deletions of targeted genes in C. albicans; the exact mechanism (trisomy followed by chromosome loss versus gene conversion) has not been determined. UAU1 insertion into the essential ORC1 gene resulted in a large proportion of trisomic strains, while gene conversion events predominated when UAU1 was inserted into the non-essential LRO1 gene. Therefore, induced aneuploidies can be used to generate HapMaps, which are essential for analyzing genome alterations and mitotic recombination events in this clonal organism. © 2008 Legrand et al.
Spending Fast and Furious: Political Advertising in 2020
Date: 2021-03-01
Creator: Travis N. Ridout, Erika Franklin Fowler, Michael M. Franz
Access: Open access
- This article is a "first look"at political advertising in 2020. Spending on political advertising in the United States in 2020 obliterated records, and Democrats held huge advantages in the presidential race and in most congressional and senatorial races. In addition, all indicators suggest that spending on digital advertising continued to rise. Political advertising was largely similar in tone to past years and, in the presidential race, was substantially more positive than 2016. In addition, interest groups remained heavily involved in federal races in 2020, airing more ads than ever before, though their spending as a percentage of total ad spending was slightly less than in 2016. Political ad spending in 2020 may have been historically high because of the impact of COVID-19 on how campaigns could reach voters, suggesting that paid advertising may decline in 2022 and 2024, at least as a percentage of total election spending.
Carbon and hydrogen isotopic composition of methane over the last 1000 years
Date: 2009-10-01
Creator: J. A. Mischler, T. A. Sowers, R. B. Alley, M. Battle, J. R., McConnell, L. Mitchell, T. Popp, E. Sofen, M. K. Spencer
Access: Open access
- New measurements of the carbon and hydrogen isotopic ratios of methane (δ13 C of CH4 and δD of CH4) over the last millennium are presented from the WAIS Divide, Antarctica, ice core (WDC05A), showing significant changes that likely were the result of human influences prior to the industrial revolution (at least as early as the 16th century of the common era (CE)). The (δ13C of CH4 data corroborate the record from Law Dome, Antarctica, with high fidelity. The new δD of CH4 data set covaries with the (δ13C of CH4 record. Both (δ13C of CH4 and δD of CH4 were relatively stable and close to the present-day values from ∼to ∼CE. Both isotopic ratios decreased to minima around 1700 CE, remained low until the late 18th century, and then rose exponentially to present-day values. Our new δD of CH4 data provide an additional independent constraint for evaluating possible CH4 source histories. We searched a broad range of source scenarios using a simple box model to identify histories consistent with the constraints of the CH 4 concentration and isotope data from 990-1730 CE. Results typically show a decrease over time in the biomass-burning source (found in 85% of acceptable scenarios) and an increase in the agricultural source (found in 77% of acceptable scenarios), indicating preindustrial human influence on atmospheric methane as proposed in previous studies. © 2009 by the American Geophysical Union.
Higgs-regularized three-loop four-gluon amplitude in N = 4 SYM: Exponentiation and Regge limits
Date: 2010-01-01
Creator: Johannes M. Henn, Stephen G. Naculich, Howard J. Schnitzer, Marcus Spradlin
Access: Open access
- We compute the three-loop contribution to the N = 4 supersymmetric Yang- Mills planar four-gluon amplitude using the recently-proposed Higgs IR regulator of Alday, Henn, Plefka, and Schuster. In particular, we test the proposed exponential ansatz for the four-gluon amplitude that is the analog of the BDS ansatz in dimensional regularization. By evaluating our results at a number of kinematic points, and also in several kinematic limits, we establish the validity of this ansatz at the three-loop level. We also examine the Regge limit of the planar four-gluon amplitude using several different IR regulators: dimensional regularization, Higgs regularization, and a cutoff regularization. In the latter two schemes, it is shown that the leading logarithmic (LL) behavior of the amplitudes, and therefore the lowest-order approximation to the gluon Regge trajectory, can be correctly obtained from the ladder approximation of the sum of diagrams. In dimensional regularization, on the other hand, there is no single dominant set of diagrams in the LL approximation. We also compute the NLL and NNLL behavior of the L-loop ladder diagram using Higgs regularization. © SISSA 2010.
Tapping at the Windows: A Collection This record is embargoed.
- Embargo End Date: 2025-05-14
Date: 2020-01-01
Creator: Samuel Milligan
Access: Embargoed
Performing Sor Juana: Reimagining a Mexican Literary Figure in the 21st Century
Date: 2020-01-01
Creator: Uriel López-Serrano
Access: Open access
- Sor Juana Inés de la Cruz (c. 1648-1695) was a Mexican nun, poet, playwright, and scholar from the colonial era. She has become an icon for various global, social, and political movements. This project looks at four dramatic works created by Sorjuanistas who reimagine Sor Juana’s story for contemporary audiences living in the United States. The works included in this essay are Estela Portillo-Trambley’s Sor Juana (1986), Karen Zacarías’s The Sins of Sor Juana (2001), and Alicia Gaspar de Alba’s “Interview with Sor Juana Inés de la Cruz” (1998/2014) and her newest work, Juana: An Opera in Two Acts (2019), libretto by Carla Lucero. In addition to reimagining Sor Juana’s story, these dramatic works expose the sexism, racism, and xenophobia perpetuated by U.S institutions of power that discriminate against Latin@ and Chican@ individuals. By shedding light on the social injustices that existed during the colonial era, an embodied Sor Juana teaches audiences how to resist and mobilize against such oppressive powers. Sor Juana’s narrative on stage is necessary because she is a role model for Latin@s/Chican@s. Sorjuanistas remind us that the body can be used to retell the narratives of the silenced individuals who are victims of oppression. By developing heritage performances, Sorjuanistas challenge histories that silence and overlook social injustices. Witnessing Sor Juana on stage triggers emotional responses to the past which allow historical actors to obtain intellectual, emotional, and political agency in an effort to affirm and remember particular contemporary and future commitments to fighting social injustices.
Do Infant Temperament Characteristics Predict Core Academic Abilities in Preschool-Aged Children?
Date: 2016-01-01
Creator: Maria A. Gartstein, Samuel P. Putnam, Rachel Kliewer
Access: Open access
- Examined relationships between temperament, measured via parent report at 4 months and structured laboratory observations at 12 months of age, and a school readiness battery administered at about 4 years of age (N =31). Scores on the School Readiness Assessment of the Bracken Basic Concept Scale (BBCS) were related to infant Positive Affectivity/Surgency (PAS), with infants described as demonstrating higher levels of PAS at 4 months of age later demonstrating greater school readiness in the domains of color, letter, and number skills. Regulatory Capacity/Orienting (RCO) at 4 months also predicted color skills, with more regulated infants demonstrating superior pre-academic functioning in this area. Analyses involving laboratory observations of temperament provided additional information concerning the importance of infant Positive Affectivity/Surgency, predictive of letter skills and overall school-readiness scores later in childhood. Results are discussed in the context of implications for theory and research, as well as early education settings.