Showing 1051 - 1100 of 7462 Items
Related neuropeptides use different balances of unitary mechanisms to modulate the cardiac neuromuscular system in the American lobster, Homarus americanus
Date: 2015-01-01
Creator: Patsy S. Dickinson, Andrew Calkins, Jake S. Stevens
Access: Open access
- To produce flexible outputs, neural networks controlling rhythmic motor behaviors can be modulated at multiple levels, including the pattern generator itself, sensory feedback, and the response of the muscle to a given pattern of motor output. We examined the role of two related neuropeptides, GYSDRNYLRFamide (GYS) and SGRNFLRFamide (SGRN), in modulating the neurogenic lobster heartbeat, which is controlled by the cardiac ganglion (CG). When perfused though an isolated whole heart at low concentrations, both peptides elicited increases in contraction amplitude and frequency. At higher concentrations, both peptides continued to elicit increases in contraction amplitude, but GYS caused a decrease in contraction frequency, while SGRN did not alter frequency. To determine the sites at which these peptides induce their effects, we examined the effects of the peptides on the periphery and on the isolated CG. When we removed the CG and stimulated the motor nerve with constant bursts of stimuli, both GYS and SGRN increased contraction amplitude, indicating that each peptide modulates the muscle or the neuromuscular junction. When applied to the isolated CG, neither peptide altered burst frequency at low peptide concentrations; at higher concentrations, SGRN decreased burst frequency, whereas GYS continued to have no effect on frequency. Together, these data suggest that the two peptides elicit some of their effects using different mechanisms; in particular, given the known feedback pathways within this system, the importance of the negative (nitric oxide) relative to the positive (stretch) feedback pathways may differ in the presence of the two peptides.
Prediction of a neuropeptidome for the eyestalk ganglia of the lobster Homarus americanus using a tissue-specific de novo assembled transcriptome
Date: 2017-03-01
Creator: Andrew E. Christie, Vittoria Roncalli, Matthew C. Cieslak, Micah G. Pascual, Andy, Yu, Tess J. Lameyer, Meredith E. Stanhope, Patsy S. Dickinson
Access: Open access
- In silico transcriptome mining is a powerful tool for crustacean peptidome prediction. Using homology-based BLAST searches and a simple bioinformatics workflow, large peptidomes have recently been predicted for a variety of crustaceans, including the lobster, Homarus americanus. Interestingly, no in silico studies have been conducted on the eyestalk ganglia (lamina ganglionaris, medulla externa, medulla interna and medulla terminalis) of the lobster, although the eyestalk is the location of a major neuroendocrine complex, i.e., the X-organ-sinus gland system. Here, an H. americanus eyestalk ganglia-specific transcriptome was produced using the de novo assembler Trinity. This transcriptome was generated from 130,973,220 Illumina reads and consists of 147,542 unique contigs. Eighty-nine neuropeptide-encoding transcripts were identified from this dataset, allowing for the deduction of 62 distinct pre/preprohormones. Two hundred sixty-two neuropeptides were predicted from this set of precursors; the peptides include members of the adipokinetic hormone-corazonin-like peptide, allatostatin A, allatostatin B, allatostatin C, bursicon α, CCHamide, corazonin, crustacean cardioactive peptide, crustacean hyperglycemic hormone (CHH), CHH precursor-related peptide, diuretic hormone 31, diuretic hormone 44, eclosion hormone, elevenin, FMRFamide-like peptide, glycoprotein hormone α2, glycoprotein hormone β5, GSEFLamide, intocin, leucokinin, molt-inhibiting hormone, myosuppressin, neuroparsin, neuropeptide F, orcokinin, orcomyotropin, pigment dispersing hormone, proctolin, pyrokinin, red pigment concentrating hormone, RYamide, short neuropeptide F, SIFamide, sulfakinin, tachykinin-related peptide and trissin families. The predicted peptides expand the H. americanus eyestalk ganglia neuropeptidome approximately 7-fold, and include 78 peptides new to the lobster. The transcriptome and predicted neuropeptidome described here provide new resources for investigating peptidergic signaling within/from the lobster eyestalk ganglia.
Distinct or shared actions of peptide family isoforms: II. Multiple pyrokinins exert similar effects in the lobster stomatogastric nervous system
Date: 2015-09-01
Creator: Patsy S. Dickinson, Sienna C. Kurland, Xuan Qu, Brett O. Parker, Anirudh, Sreekrishnan, Molly A. Kwiatkowski, Alex H. Williams, Alexandra B. Ysasi, Andrew E. Christie
Access: Open access
- Many neuropeptides are members of peptide families, with multiple structurally similar isoforms frequently found even within a single species. This raises the question of whether the individual peptides serve common or distinct functions. In the accompanying paper, we found high isoform specificity in the responses of the lobster (Homarus americanus) cardiac neuromuscular system to members of the pyrokinin peptide family: only one of five crustacean isoforms showed any bioactivity in the cardiac system. Because previous studies in other species had found little isoform specificity in pyrokinin actions, we examined the effects of the same five crustacean pyrokinins on the lobster stomatogastric nervous system (STNS). In contrast to our findings in the cardiac system, the effects of the five pyrokinin isoforms on the STNS were indistinguishable: they all activated or enhanced the gastric mill motor pattern, but did not alter the pyloric pattern. These results, in combination with those from the cardiac ganglion, suggest that members of a peptide family in the same species can be both isoform specific and highly promiscuous in their modulatory capacity. The mechanisms that underlie these differences in specificity have not yet been elucidated; one possible explanation, which has yet to be tested, is the presence and differential distribution of multiple receptors for members of this peptide family.
Investigating the role of eyes absent in photoreceptor axon targeting in Drosophila melanogaster
Date: 2021-01-01
Creator: Bethany J. Thach
Access: Open access
- The eya gene is essential to development of the Drosophila visual system and eye-specific loss of function mutations in the gene commonly result in the missing eye phenotype. The eya2m35g mutation carries a deletion of exon 1B and adjacent regulatory sequences. Flies carrying the eya2m35g allele exhibit a photoreceptor axon phenotype that has not previously been associated with the eya gene. To determine a potential role for eya in photoreceptor axon targeting, I characterized various phenotypes of eya2m35g mutants and generated additional eya alleles consisting of smaller deletions within the eya2m35g mutation to locate the genetic source of axonal disruption. Using immunofluorescence staining to visualize Eya protein, I found a loss of eya expression in the optic lobe region of eya2m35g stage 9 embryos and third instar larvae. I also observed a loss of retinal basal glial (RBG) cells in the larval eye disc. Finally, I demonstrated that the disconnected axon phenotype is generated when a region of the intron immediately downstream of exon 1B is deleted. These findings suggest that a possible regulatory element for eya that is essential for photoreceptor axon targeting exists in this intronic region.
Acute effects of sex steroids on visual processing in male goldfish
Date: 2018-01-01
Creator: S. Yue, V. Wadia, N. Sekula, P. S. Dickinson, R. R., Thompson
Access: Open access
- Elevations of sex steroids induced by social cues can rapidly modulate social behavior, but we know little about where they act within the nervous system to produce such effects. In male goldfish, testosterone (T) rapidly increases approach responses to the visual cues of females through its conversion to estradiol. Because aromatase is expressed in the retina, we tested if T can acutely influence retina responses to visual stimuli, and investigated the receptor mechanisms that may mediate such effects. Specifically, we measured FOS protein immunoreactivity to determine if T affects cellular responses to visual stimuli that include females, and used electrophysiology to investigate whether T can generally affect light sensitivity. We found that T acutely increased FOS responses to the simultaneous onset of light and the presence of female visual stimuli, both of which would normally be associated with early morning spawning, and increased electrophysiological responses to low intensity light pulses. Both effects were blocked by an estrogen receptor beta (ERβ) antagonist, indicating that T is likely being converted to estradiol (E2) and acting through an ERβ mediated mechanism to acutely modulate visual processing. Changes in sensory processing could subsequently influence approach behavior to increase reproductive success in competitive mating environments.
C-terminal methylation of truncated neuropeptides: An enzyme- assistedextraction artifact involving methanol
Date: 2013-01-01
Creator: Elizabeth A. Stemmler, Elizabeth E. Barton, Onyinyechi K. Esonu, Daniel A. Polasky, Laura L., Onderko, Audrey B. Bergeron, Andrew E. Christie, Patsy S. Dickinson
Access: Open access
- Neuropeptides are the largest class of signaling molecules used by nervous systems. Today, neuropeptidediscovery commonly involves chemical extraction from a tissue source followed by mass spectrometriccharacterization. Ideally, the extraction procedure accurately preserves the sequence and any inher-ent modifications of the native peptides. Here, we present data showing that this is not always true.Specifically, we present evidence showing that, in the lobster Homarus americanus, the orcokinin fam-ily members, NFDEIDRSGFG-OMe and SSEDMDRLGFG-OMe, are non-native peptides generated fromfull-length orcokinin precursors as the result of a highly selective peptide modification (peptide trun-cation with C-terminal methylation) that occurs during extraction. These peptides were observed byMALDI-FTMS and LC-Q-TOFMS analyses when eyestalk ganglia were extracted in a methanolic solvent,but not when tissues were dissected, co-crystallized with matrix, and analyzed directly with methanolexcluded from the sample preparation. The identity of NFDEIDRSGFG-OMe was established using MALDI-FTMS/SORI-CID, LC-Q-TOFMS/MS, and comparison with a peptide standard. Extraction substitutingdeuterated methanol for methanol confirmed that the latter is the source of the C-terminal methyl group,and MS/MS confirmed the C-terminal localization of the added CD3. Surprisingly, NFDEIDRSGFG-OMe isnot produced via a chemical acid-catalyzed esterification. Instead, the methylated peptide appears toresult from proteolytic truncation in the presence of methanol, as evidenced by a reduction in conver-sion with the addition of a protease-inhibitor cocktail; heat effectively eliminated the conversion. Thisunusual and highly specific extraction-derived peptide conversion exemplifies the need to consider bothchemical and biochemical processes that may modify the structure of endogenous neuropeptides. © 2013 The Authors. Published by Elsevier Inc. All rights reserved.
The quantitative genetics of incipient speciation: Heritability and genetic correlations of skeletal traits in populations of diverging favia fragum ecomorphs
Date: 2011-12-01
Creator: David B. Carlon, Ann F. Budd, Catherine Lippé, Rose L. Andrew
Access: Open access
- Recent speciation events provide potential opportunities to understand the microevolution of reproductive isolation. We used a marker-based approach and a common garden to estimate the additive genetic variation in skeletal traits in a system of two ecomorphs within the coral species Favia fragum: a Tall ecomorph that is a seagrass specialist, and a Short ecomorph that is most abundant on coral reefs. Considering both ecomorphs, we found significant narrow-sense heritability (h 2) in a suite of measurements that define corallite architecture, and could partition additive and nonadditive variation for some traits. We found positive genetic correlations for homologous height and length measurements among different types of vertical plates (costosepta) within corallites, but negative correlations between height and length within, as well as between costosepta. Within ecomorphs, h 2 estimates were generally lower, compared to the combined ecomorph analysis. Marker-based estimates of h 2 were comparable to broad-sense heritability (H) obtained from parent-offspring regressions in a common garden for most traits, and similar genetic co-variance matrices for common garden and wild populations may indicate relatively small G × E interactions. The patterns of additive genetic variation in this system invite hypotheses of divergent selection or genetic drift as potential evolutionary drivers of reproductive isolation. © 2011 The Author(s). Evolution© 2011 The Society for the Study of Evolution.
Characterizing the influence of Atlantic water intrusion on water mass formation and primary production in Kongsfjorden, Svalbard
Date: 2015-05-01
Creator: Courtney Michelle Payne
Access: Open access
- With warming global temperatures and changes to large-scale ocean circulation patterns, warm water intrusion into Arctic fjords is increasingly affecting fragile polar ecosystems. This study investigated how warm Atlantic water intrusion and the tidewater glacial melting it causes impacted water mass formation and primary productivity in Kongsfjorden, Svalbard. Data were collected over a 2-week period during the height of the melt season in August near the Kronebreen/Kongsvegen glacier complex, the most rapidly retreating glacier in Spitsbergen. Since 1998, intruding waters have warmed between 4 and 5.5˚C, which has prevented sea ice formation and changed the characteristics of fjord bottom waters. Increased glacial melting in the last decade has changed the characteristics of surface waters in the fjord. Modeled light fields suggest that suspended sediment in this glacial meltwater has reduced the euphotic zone close to the ice face, preventing high primary production in both the consistent and intermittent sediment-laden meltwater plumes. However, measurements collected close to terrestrially terminating glaciers indicate that extremely high primary production can occur in conditions of low turbidity. The results of this study support a three-part model of the effects of warm-water intrusion on water mass formation and primary production, where changes in sea ice coverage and tidewater glacial dynamics affect the optical light field. This model allows for spatial predictions for the most likely impacts of warm water intrusion on primary production in Spitsbergen, and could be extrapolated out to explore potential phytoplankton response in other regions susceptible to warm-water intrusion.
The neuromuscular transform of the lobster cardiac system explains the opposing effects of a neuromodulator on muscle output
Date: 2013-10-18
Creator: Alex H. Williams, Andrew Calkins, Timothy O'Leary, Renee Symonds, Eve, Marder, Patsy S. Dickinson
Access: Open access
- Motor neuron activity is transformed into muscle movement through a cascade of complex molecular and biomechanical events. This nonlinear mapping of neural inputs to motor behaviors is called the neuromuscular transform (NMT). We examined the NMT in the cardiac system of the lobster Homarus americanus by stimulating a cardiac motor nerve with rhythmic bursts of action potentials and measuring muscle movements in response to different stimulation patterns. The NMT was similar across preparations, which suggested that it could be used to predict muscle movement from spontaneous neural activity in the intact heart. We assessed this possibility across semi-intact heart preparations in two separate analyses. First, we performed a linear regression analysis across 122 preparations in physiological saline to predict muscle movements from neural activity. Under these conditions, the NMT was predictive of contraction duty cycle but was unable to predict contraction amplitude, likely as a result of uncontrolled interanimal variability. Second, we assessed the ability of the NMT to predict changes in motor output induced by the neuropeptide C-type allatostatin. Wiwatpanit et al. (2012) showed that bath application of C-type allatostatin produced either increases or decreases in the amplitude of the lobster heart contractions. We show that an important component of these preparation-dependent effects can arise from quantifiable differences in the basal state of each preparation and the nonlinear form of the NMT. These results illustrate how properly characterizing the relationships between neural activity and measurable physiological outputs can provide insight into seemingly idiosyncratic effects of neuromodulators across individuals. © 2013 the authors.
Making Human Beings and Citizens: The Educational Philosophies of Jean-Jacques Rousseau and Mary Wollstonecraft Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2021-01-01
Creator: Mollie Claire Eisner
Access: Access restricted to the Bowdoin Community
The Jewish “Other” in Argentina: Antisemitism, Exclusion, and the Formation of Argentine Nationalism and Identity in the 20th Century and during Military Rule (1976-1983)
Date: 2021-01-01
Creator: Marcus Helble
Access: Open access
- Throughout the 20th century, Argentine leaders and social actors attempted to shape distinct national identities and a sense of nationalism that corresponded to their respective political ideologies. Beginning in the first couple decades of the 20th century, the formation of a Jewish “other” would be central to the construction of both Argentine national identity and nationalism. This thesis argues that the military dictatorship that led the country from 1976 to 1983 built on this othering of the Jewish community as military leaders sought to forge a national identity linked to Catholicism. It focuses first on three separate periods of the early and mid-20th centuries and how governments in that period built, maintained, and altered the view of the Jewish community as a not fully Argentine “other” living in the country. Using several editions of a far-right antisemitic periodical, declassified State Department documents, and testimonies of Jewish political prisoners and soldiers, the thesis transitions to focus on the military dictatorship of the 1970s and 1980s. It examines two separate periods of the dictatorship, highlighting first the role antisemitic beliefs, and opposing such views, had in an internal power struggle within the military government. In the second period of the dictatorship, during the Malvinas (Falklands) War, the thesis examines how antisemitism became a central part of the military’s efforts to consolidate a sense of national unity during the conflict, even as Jews participated largely for the first time within Argentine nationalism and the military.
The peptide hormone pQDLDHVFLRFamide (crustacean myosuppressin) modulates the Homarus americanus cardiac neuromuscular system at multiple sites
Date: 2009-12-15
Creator: J. S. Stevens, C. R. Cashman, C. M. Smith, K. M. Beale, D. W., Towle, A. E. Christie, P. S. Dickinson
Access: Open access
- pQDLDHVFLRFamide is a highly conserved crustacean neuropeptide with a structure that places it within the myosuppressin subfamily of the FMRFamide-like peptides. Despite its apparent ubiquitous conservation in decapod crustaceans, the paracrine and/or endocrine roles played by pQDLDHVFLRFamide remain largely unknown. We have examined the actions of this peptide on the cardiac neuromuscular system of the American lobster Homarus americanus using four preparations: the intact animal, the heart in vitro, the isolated cardiac ganglion (CG), and a stimulated heart muscle preparation. In the intact animal, injection of myosuppressin caused a decrease in heartbeat frequency. Perfusion of the in vitro heart with pQDLDHVFLRFamide elicited a decrease in the frequency and an increase in the amplitude of heart contractions. In the isolated CG, myosuppressin induced a hyperpolarization of the resting membrane potential of cardiac motor neurons and a decrease in the cycle frequency of their bursting. In the stimulated heart muscle preparation, pQDLDHVFLRFamide increased the amplitude of the induced contractions, suggesting that myosuppressin modulates not only the CG, but also peripheral sites. For at least the in vitro heart and the isolated CG, the effects of myosuppressin were dose-dependent (10 -9 to 10-6mol l-1 tested), with threshold concentrations (10-8-10-7 mol l-1) consistent with the peptide serving as a circulating hormone. Although cycle frequency, a parameter directly determined by the CG, consistently decreased when pQDLDHVFLRFamide was applied to all preparation types, the magnitudes of this decrease differed, suggesting the possibility that, because myosuppressin modulates the CG and the periphery, it also alters peripheral feedback to the CG.
Identification, physiological actions, and distribution of TPSGFLGMRamide: A novel tachykinin-related peptide from the midgut and stomatogastric nervous system of Cancer crabs
Date: 2007-06-01
Creator: Elizabeth A. Stemmler, Braulio Peguero, Emily A. Bruns, Patsy S. Dickinson, Andrew E., Christie
Access: Open access
- In most invertebrates, multiple species-specific isoforms of tachykinin-related peptide (TRP) are common. In contrast, only a single conserved TRP isoform, APSGFLGMRamide, has been documented in decapod crustaceans, leading to the hypothesis that it is the sole TRP present in this arthropod order. Previous studies of crustacean TRPs have focused on neuronal tissue, but the recent demonstration of TRPs in midgut epithelial cells in Cancer species led us to question whether other TRPs are present in the gut, as is the case in insects. Using direct tissue matrix assisted laser desorption/ionization Fourier transform mass spectrometry, in combination with sustained off-resonance irradiation collision-induced dissociation, we found that at least one additional TRP is present in Cancer irroratus, Cancer borealis, Cancer magister, and Cancer productus. The novel TRP isoform, TPSGFLGMRamide, was present not only in the midgut, but also in the stomatogastric nervous system (STNS). In addition, we identified an unprocessed TRP precursor APSGFLGMRG, which was detected in midgut tissues only. TRP immunohistochemistry, in combination with preadsorption studies, suggests that APSGFLGMRamide and TPSGFLGMRamide are co-localized in the stomatogastric ganglion (STG), which is contained within the STNS. Exogenous application of TPSGFLGMRamide to the STG elicited a pyloric motor pattern that was identical to that elicited by APSGFLGMRamide, whereas APSGFLGMRG did not alter the pyloric motor pattern. © 2007 The Authors.
All That Influences the Condition of Women: The Moral Foundations of Female Education in Tocqueville and Rousseau This record is embargoed.
- Embargo End Date: 2026-05-20
Date: 2021-01-01
Creator: Nicole Danielle Tjin A Djie
Access: Embargoed
Caring like a state: The elaboration of a care ideology in Peru and Sri Lanka in the 20th century
Date: 2015-05-01
Creator: Katharine Herman
Access: Open access
- This project compares the interaction between the government and the population in both Peru and Sri Lanka through the 20th century, focusing on the provision of care (services, benefits, and recognition as provided by the governing apparatus) as a locus for their most meaningful interaction. The provision of care can be seen as a form of communication established in certain practices, symbols, and discourses. Moreover, the provision of care works to reorient the subject population into a more beneficial relationship with the state–notably one of increased dependence and trust. Through the elaboration of what care is and how it functions, a care ideology is established creating a terrain through which the politics of the governed and the governing alike can be legitimized. Care ideology can then be considered a further articulation of the state idea, creating a dialogue about how governance can or should be affected within a certain context. These case studies then illustrate the terrain over which both of these governing bodies work. In Sri Lanka, the governing apparatus provides a means for citizens to live a symbolically meaningful life through paddy farming. In Peru, the governing apparatus provides access to the state through civil infrastructure like roads and education. Through care, material goods become invested with meaning and the state idea becomes materialized. Care is a then substantial project undertaken by the state to ensure its own reproduction and further widen the possibilities for the state project to be effected through its own citizens.
Accretion Onto a Black Hole at the Center of a Neutron Star: Nuclear Equations of State
Date: 2022-01-01
Creator: Sophia Christina Schnauck
Access: Open access
- A recent re-examination of Bondi accretion (see Richards, Baumgarte and Shapiro (2021)) revealed that, for stiff equations of state (EOSs), steady-state accretion can only occur for accretion rates exceeding a certain minimum. To date, this result has been explored only for gamma-law equations of state. Instead, we consider accretion onto a small black hole residing at the center of a neutron star governed by a more realistic nuclear EOS. We generalize the relativistic Bondi solution for such EOSs, approximated by piecewise polytropes, and thereby obtain analytical expressions for the accretion rates which were reflected in our numerical simulations. After taking several different piecewise EOSs at different neutron star densities into account, the accretion rates of the different EOSs were only slightly larger than the previously observed minimum. In other words there appears to be evidence for a nearly universal accretion rate that depends only on the black hole mass. However, we also observed that for certain densities the fluid profiles of several EOSs exhibited superluminal sound speeds outside the horizon of the black hole, suggesting that the EOSs are not appropriate at these densities.
Evaluating Dam Relicensing and River Herring Habitat Restoration from a Broad, Multi-Ecosystem Perspective
Date: 2022-01-01
Creator: Matthew L. Thomas
Access: Open access
- This study investigates the potential benefits of using a broad, multi-ecosystem analysis in the licensing and relicensing of hydropower facilities. Specifically, it considers the impact of river herring restoration on coastal food webs and cod and other groundfish populations in the Gulf of Maine. The past two decades of research on fisheries management, ecosystem connectivity, and the connection between river herring and groundfish in the Gulf of Maine have resulted in a better understanding of the ways in which human activities, such as dam building, influence ecological processes. The paper analyzes two case studies of six Maine dams currently engaged in the Federal Energy Regulatory Commission’s (FERC) hydroelectric dam relicensing process. The analysis illustrates the shortcomings of the Federal Power Act’s provisions that address the balancing of ecological and power generation concerns. Following the case studies, a series of policy recommendations are presented to encourage a more transparent and predictable relicensing process that adequately values both ecological and power generation goals. Changes are suggested for both the FERC process itself and the process by which state and federal resource agencies may provide comments regarding how a proposed dam licensing or relicensing affects natural resources under their jurisdiction. The proposed policy recommendations will increase the resilience of natural systems as they adapt to climate impacts.
An Investigation on Data Gaps in Scope 3 Emissions Accounting and Disclosure using 2010-2021 Carbon Disclosure Project (CDP) Questionnaire Responses This record is embargoed.
- Embargo End Date: 2027-05-17
Date: 2022-01-01
Creator: Samara Nassor
Access: Embargoed
Antimicrobial Compounds in the Lobster, Homarus americanus: An Investigation of Lobster Shell Extracts This record is embargoed.
- Embargo End Date: 2027-05-19
Date: 2022-01-01
Creator: Usira Ahmed Ali
Access: Embargoed
Bowdoin College Catalogue (1975-1976)
Date: 1976-01-01
Access: Open access
- Bowdoin College Bulletin no. 398
“Unstuck in Time and Space”: Time Travels in Teen Cinema Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2021-01-01
Creator: Hallowell Lyne
Access: Access restricted to the Bowdoin Community
Chromatin-conformation differences in natural populations of D. melanogaster Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2026-06-01
Date: 2021-01-01
Creator: Nicholas J. Purchase
Access: Access restricted to the Bowdoin Community
The Photocatalytic Degradation of Ibuprofen and Atenolol Using Bismuth Oxychloride and Titanium Dioxide
Date: 2021-01-01
Creator: Kamyron Anthony Speller
Access: Open access
- Pharmaceuticals and personal care products (PPCPs) are contaminating natural bodies of water and are problematic for aquatic organisms and ecosystems. Generally, PPCPs are introduced to water systems due to incomplete removal by wastewater treatment plants (WWTPs). As such, it is vital to find ways to remediate these problematic contaminants before they are discharged into the environment. In this study, two photocatalysts¾titanium dioxide (TiO2) and bismuth oxychloride (BiOCl)¾were compared to determine their relative efficiencies (degradation rates) and dominant degradation mechanism (hydroxyl radical production or direct oxidation) with the goal of photocatalytically degrading two pharmaceuticals, atenolol and ibuprofen, using UV (254 nm) light. While TiO2 has been used extensively for photocatalytic degradation, BiOCl is a newer photocatalyst. The two pharmaceuticals selected for study represent two large classes of drugs (aryloxypropanolamine and propionic acid derivatives, respectively) that have been detected in the influent into and effluent from wastewater treatment plants and in the environment. When irradiated at 254 nm, BiOCl degrades ibuprofen with a rate constant 15 times greater than TiO2. On the other hand, TiO2 degrades atenolol with a rate constant 2.2 times greater than BiOCl. LCMS analysis of photodegradation products reveals different products produced by the two photocatalysts, providing evidence for the dominance of different degradation mechanisms for the two photocatalysts. In summary, this work suggests that BiOCl, potentially used in combination with TiO2, holds potential for degrading PPCPs in natural bodies of water.
Bowdoin College Catalogue (1973-1974)
Date: 1974-01-01
Access: Open access
- Bowdoin College Bulletin no. 390
Palimpsestuous London: Spatial and Temporal Layering in Fin-de-Siècle Victorian Fiction Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2015-05-01
Creator: Elisabeth A Strayer
Access: Access restricted to the Bowdoin Community
Semaphorin-Induced Plasticity in the Nervous System of the Cricket, Gryllus bimaculatus
Date: 2021-01-01
Creator: Alicia G. Edwards
Access: Open access
- The adult auditory system of the cricket, Gryllus bimaculatus, exhibits a rare example of neuronal plasticity. Upon deafferentation, we observe medial dendrites that normally respect the midline of the PTG in the central nervous system sprouting across the boundary and forming synaptic connections with the contralateral auditory afferents. The Horch Lab has investigated key molecular factors that might play a causal role in this paradigm. Specifically, the protein Sema1a.2 comes from a guidance molecule family and has a role in developmental neuronal plasticity in other organisms. In this study, I explored the role of Sema1a.2 in the neuronal plasticity of the adult auditory system of the cricket by conducting a series of dsRNA knockdown experiments targeting Sema1a.2 followed by backfill procedures in which we iontophoresed dye into the Ascending Neurons (ANs) to visualize the anatomical effects of the knockdown experiments using confocal microscopy. We found that there were no significant differences between animals injected with dsRNA against GFP and Sema1a.2 volume, with respect to qualitative and quantitative data. However, we believe with an increase in cohort size, the trends observed, particularly the effect of Sema1a.2 knockdowns on CWM and CBM volumes, will become more pronounced and significant. Potential future pathways could include conducting double knockdowns of Sema1a.2 and Sema2a to observe if these two proteins are working together to create a more obvious effect on midline crossing and branching. Other options also include looking into other protein families that might be the causing factor in this rare phenomenon (toll-like receptors).
Characterization of Yellow Family Proteins in Gryllus bimaculatus
Date: 2021-01-01
Creator: Alexandra W. Rubenstein
Access: Open access
- Neuronal plasticity occurs in developing nervous systems, with adult organisms rarely able to recover from neurological damage. The cricket, Gryllus bimaculatus, is useful to study neuronal plasticity due to its reorganization of the auditory system in response to injury beyond development. When a cricket ear is removed and auditory afferents severed, a rare phenomenon occurs: the dendrites of interneurons on the deafferented side cross the typically-respected midline of the prothoracic ganglion to form functional synapses with auditory afferents from the opposite side. To find proteins involved in this phenomenon, the Horch Lab assembled a de novo transcriptome from neurons in the prothoracic ganglion of G. bimaculatus. Differential gene expression analysis revealed upregulated protein yellows post-deafferentation, indicating these proteins could influence neuronal plasticity in the adult cricket CNS. I focused on characterizing the protein yellow family in the cricket. By relating protein yellows evolutionarily, mapping them onto the genome, and analyzing their sequences, I discovered the cricket has 10 yellow genes, including a newly identified yellow-r* and a block of yellows showing synteny with insect genomes. Additionally, yellow-e and -x in crickets are closely related to bacterial yellow, perhaps indicating a role for horizontal gene transfer in yellow gene evolution. The protein upregulated in the cricket CNS is closely related with yellow-f’s in other insects, indicating yellow-f is likely a secreted protein, highly expressed in the CNS, multifunctional, and conserved across insects. Characterizing yellow-f can give insight into how these upregulated proteins might be related to neuronal plasticity in G. bimaculatus.
Jigs, Reels, and “Realness”: An Investigation of Ideas of Authenticity and Tradition in New England French Canadian Music
Date: 2021-01-01
Creator: Lowell Ruck
Access: Open access
- Franco-American culture is increasingly recognized as an integral part of the heritage of Maine and New England, and has attracted growing academic attention in recent years. But while many scholars and cultural promoters focus on the French language in their work on this subject, few studies have considered the position of traditional music in Franco-American communities in the 21st century. This thesis examines French Canadian traditional music as it is played in New England and the ways in which musicians think about authenticity and tradition in their art. Using material from ethnographic interviews, it illuminates how musicians draw from individual, familial, and communal experiences and from past, present, and future conceptions of authentic tradition in their roles as cultural mediators. Ultimately, it suggests that players of French Canadian traditional music interact with this tradition in diverse ways, and that in doing so, they help to maintain the vibrancy of Franco-American cultural practices. La culture franco-américaine est reconnue de plus en plus comme partie intégrante de l’héritage du Maine et de la Nouvelle-Angleterre et attire maintenant l’attention académique. Mais même si beaucoup d’érudits et de promoteurs culturels ciblent la langue française dans leur travail sur ce sujet, peu d’études ont considéré la position de la musique traditionnelle dans les communautés franco-américaines du XXIe siècle. Cette thèse se concentre sur la musique traditionnelle canadienne-française de la Nouvelle-Angleterre et sur les façons dont les musiciens conceptualisent l’authenticité et la tradition dans leur art. En utilisant des entrevues ethnographiques, elle illumine comment les musiciens tirent des expériences individuelles, familiales et communautaires et des idées du passé, du présent et de l’avenir dans leurs rôles comme médiateurs culturels. Finalement, elle suggère que les joueurs de la musique traditionnelle canadienne-française interagissent avec cette tradition de plusieurs façons, et ce faisant, aident à maintenir la brillance des pratiques culturelles franco-américaines.
Possessing Her: Embodying Identity in Exorcism Cinema Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2021-01-01
Creator: Alicia Echavarria
Access: Access restricted to the Bowdoin Community
Critical Phenomena in the Gravitational Collapse of Electromagnetic Dipole and Quadrupole Waves
Date: 2021-01-01
Creator: Maria F. Perez Mendoza
Access: Open access
- We report on critical phenomena in the gravitational collapse of electromagnetic waves. Generalizing earlier results that focused on dipole electromagnetic waves, we here compare with quadrupole waves in axisymmetry. We perform numerical simulations of dipole and quadrupole wave initial data, fine-tuning both sets of data to the onset of black hole formation in order to study the critical solution and related critical phenomena. We observe that different multipole moments have different symmetries, indicating that the critical solution for electromagnetic waves cannot be unique, at least not globally. This is confirmed in our numerical simulations: while dipole data lead to a single center of collapse, at the center of symmetry, quadrupole data feature two separate centers of collapse on the symmetry axis, above and below the center of symmetry -- reminiscent of similar findings reported for critical collapse of vacuum gravitational waves. While the critical solution for neither the dipole nor the quadrupole data is exactly self-similar, we find that their approximate echoing periods appear to differ, as do the critical exponents. We discuss whether the centers of collapse found for dipole and quadrupole data might all have the same properties, which would suggest a ``local uniqueness" of the critical solution. Instead, we provide some evidence -- including the differing echoing periods and critical exponents -- suggesting that the critical solutions are distinct even locally. We speculate on the implications of our findings for critical phenomena in the collapse of vacuum gravitational waves, which share with electromagnetic waves the absence of a spherically symmetric critical solution.
Hypersensitization of Helicobacter pylori to Antibiotics Through Perturbation of Bacterial Glycan Armor Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2022-01-01
Creator: William J. Rackear
Access: Access restricted to the Bowdoin Community
Illumination, Inspiration, and Inquisition: The 15th and 16th century reception and reaction of Dante’s Divina Commedia in Spain This record is embargoed.
- Embargo End Date: 2025-05-19
Date: 2022-01-01
Creator: Francesca Mauro
Access: Embargoed
Sex- and age-specific susceptibility of parvalbumin neurons to DNA methylation in a model of early life adversity This record is embargoed.
- Embargo End Date: 2025-05-19
Date: 2022-01-01
Creator: Alissa Chen
Access: Embargoed
Religious Negotiation and Identity Formation: Reading Material Religion in Oaxaca’s “Guelaguetza Oficial”
Date: 2023-01-01
Creator: Rene Sebastian Cisneros
Access: Open access
- The Oaxacan Guelaguetza Oficial is a folk-dance festival in the state of Oaxaca, Mexico which takes place on the last two Mondays of July each year. This state-sponsored celebration of Oaxacan identity is intertwined within histories of Indigenous religious belief and Catholic everyday practice. The Guelaguetza Oficial can be traced back to late 19thcentury celebrations venerating the Virgen del Carmen Alto. Oaxaqueños today predominantly practice an Indigenous-Catholic tradition whose rituals, festive scripts, pantheon of popular saints, and immanent understandings of heavenly power over earthly events can be traced back to negotiations between Indigenous forms of popular belief and institutionalized Catholic practice. Through historical and present-day religious tensions between existing modes of Indigenous religious belief and institutionalized Oaxacan Catholic practice, this thesis asserts that while Indigeneity often represented an obstacle to different structures of power in Mexican history, hegemonic institutions eventually came to accept the lasting presence of Indigenous identity and religious life to varying degrees within Mexican society and culture. This resulting reading of Guelaguetza demonstrates how religion is fundamentally implicated in the history of public festival and popular culture in Oaxaca. Furthermore, this thesis argues that Indigenous-Catholicism has not lost its prominence in public space in Oaxaca despite the reforms of post-1910 Oaxacan state and Mexican national politics and the effects of globalized neoliberal economies in the late 20th and early 21st centuries.
Timing the Paleozoic Oxygenation of the Deep Ocean Using Thallium Isotopes This record is embargoed.
- Embargo End Date: 2028-05-17
Date: 2023-01-01
Creator: Jean Nikolas R. Clemente
Access: Embargoed
Sensitivity Analysis of Basins of Attraction for Nelder-Mead
Date: 2022-01-01
Creator: Sonia K. Shah
Access: Open access
- The Nelder-Mead optimization method is a numerical method used to find the minimum of an objective function in a multidimensional space. In this paper, we use this method to study functions - specifically functions with three-dimensional graphs - and create images of the basin of attraction of the function. Three different methods are used to create these images named the systematic point method, randomized centroid method, and systemized centroid method. This paper applies these methods to different functions. The first function has two minima with an equivalent function value. The second function has one global minimum and one local minimum. The last function studied has several minima of different function values. The systematic point method is a reliable method in particular scenarios but is extremely sensitive to changes in the initial simplex. The randomized centroid method was not found to be useful as the basin of attraction images are difficult to understand. This made it particularly troublesome to know when the method was working effectively and when it was not. The systemized centroid method appears to be the most precise and effective method at creating the basin of attraction in most cases. This method rarely fails to find a minimum and is particularly adept at finding global minima more effectively compared to local minima. It is important to remember that these conclusions are simply based off the results of the methods and functions studied and that more effective methods may exist.
"In Loving Virtue": Staging the Virgin Body in Early Modern Drama
Date: 2022-01-01
Creator: Miranda Viederman
Access: Open access
- The aim of this Honors project is to investigate representations of female virginity in Renaissance English dramatic works. I view the period as one in which the womb became the site of a unique renewal of cultural anxieties surrounding the stability of the patriarchy and the inaccessibility of female sexual desire. I am most interested in virginity as a “bodily narrative” dependent on the construction and maintenance of performance. I analyze representations of virginity in female characters from four works of drama originating in the Jacobean period of the English Renaissance, during and after the end of the reign of Elizabeth I, the Virgin Queen. Across four chapters, I examine the characters of Isabella from Shakespeare’s Measure for Measure (1604), Beatrice-Joanna from Thomas Middleton’s The Changeling (1622), the Jailer’s Daughter from Shakespeare and Fletcher’s The Two Noble Kinsmen (1634), and Helen from Shakespeare’s All’s Well That Ends Well (c. 1602-1605). To establish a framework for my readings, I situate each work in its contemporary cultural context, drawing upon Catholic and Protestant religious doctrines, period medical texts, and popular culture. I intend to explore the complex, often contradictory nature of the forms of virginity the plays depict. Still, I hope by uncovering the opportunities these four characters are provided by their virginity, that I can widen the confines of the category.
#IVFgotyou: Instagram IVF Influencers as Social (Media) Support Systems
Date: 2023-01-01
Creator: Susu Gharib
Access: Open access
- This paper details the ways in which IVF and infertility influencers on Instagram use their platforms to resist the silence surrounding reproductive difficulties. The analysis draws upon a thematic analysis of posts tagged with IVF-related hashtags and a semi-structured ethnographic interview with one influencer. Through these methods, I found that influencers build intimate publics through their platforms by sharing their journeys, interacting with followers, and reciprocal support. Within the context of the intimate publics, influencers are able to connect with others who understand their experiences, allowing them to break through the silence they may feel in their offline social groups.
Differential modulation of the Homarus americanus cardiac neuromuscular system across cell types and among neuropeptide isoforms Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2020-01-01
Creator: Emily R Oleisky
Access: Access restricted to the Bowdoin Community
From local to global behavior in competitive Lotka-Volterra systems
Date: 2003-01-01
Creator: E. C. Zeeman, M. L. Zeeman
Access: Open access
- In this paper we exploit the linear, quadratic, monotone and geometric structures of competitive Lotka-Volterra systems of arbitrary dimension to give geometric, algebraic and computational hypotheses for ruling out nontrivial recurrence. We thus deduce the global dynamics of a system from its local dynamics. The geometric hypotheses rely on the introduction of a split Liapunov function. We show that if a system has a fixed point p ∈ int R+n and the carrying simplex of the system lies to one side of its tangent hyperplane at p, then there is no nontrivial recurrence, and the global dynamics are known. We translate the geometric hypotheses into algebraic hypotheses in terms of the definiteness of a certain quadratic function on the tangent hyperplane. Finally, we derive a computational algorithm for checking the algebraic hypotheses, and we compare this algorithm with the classical Volterra-Liapunov stability theorem for Lotka-Volterra systems.
Protein Transition: Alternative Proteins and Policy Pathways to a More Sustainable Diet in the United States This record is embargoed.
- Embargo End Date: 2025-05-14
Date: 2020-01-01
Creator: Anna Barnes
Access: Embargoed