Showing 21 - 30 of 63 Items
Investigating the role of eyes absent in photoreceptor axon targeting in Drosophila melanogaster
Date: 2021-01-01
Creator: Bethany J. Thach
Access: Open access
- The eya gene is essential to development of the Drosophila visual system and eye-specific loss of function mutations in the gene commonly result in the missing eye phenotype. The eya2m35g mutation carries a deletion of exon 1B and adjacent regulatory sequences. Flies carrying the eya2m35g allele exhibit a photoreceptor axon phenotype that has not previously been associated with the eya gene. To determine a potential role for eya in photoreceptor axon targeting, I characterized various phenotypes of eya2m35g mutants and generated additional eya alleles consisting of smaller deletions within the eya2m35g mutation to locate the genetic source of axonal disruption. Using immunofluorescence staining to visualize Eya protein, I found a loss of eya expression in the optic lobe region of eya2m35g stage 9 embryos and third instar larvae. I also observed a loss of retinal basal glial (RBG) cells in the larval eye disc. Finally, I demonstrated that the disconnected axon phenotype is generated when a region of the intron immediately downstream of exon 1B is deleted. These findings suggest that a possible regulatory element for eya that is essential for photoreceptor axon targeting exists in this intronic region.
The Photocatalytic Degradation of Ibuprofen and Atenolol Using Bismuth Oxychloride and Titanium Dioxide
Date: 2021-01-01
Creator: Kamyron Anthony Speller
Access: Open access
- Pharmaceuticals and personal care products (PPCPs) are contaminating natural bodies of water and are problematic for aquatic organisms and ecosystems. Generally, PPCPs are introduced to water systems due to incomplete removal by wastewater treatment plants (WWTPs). As such, it is vital to find ways to remediate these problematic contaminants before they are discharged into the environment. In this study, two photocatalysts¾titanium dioxide (TiO2) and bismuth oxychloride (BiOCl)¾were compared to determine their relative efficiencies (degradation rates) and dominant degradation mechanism (hydroxyl radical production or direct oxidation) with the goal of photocatalytically degrading two pharmaceuticals, atenolol and ibuprofen, using UV (254 nm) light. While TiO2 has been used extensively for photocatalytic degradation, BiOCl is a newer photocatalyst. The two pharmaceuticals selected for study represent two large classes of drugs (aryloxypropanolamine and propionic acid derivatives, respectively) that have been detected in the influent into and effluent from wastewater treatment plants and in the environment. When irradiated at 254 nm, BiOCl degrades ibuprofen with a rate constant 15 times greater than TiO2. On the other hand, TiO2 degrades atenolol with a rate constant 2.2 times greater than BiOCl. LCMS analysis of photodegradation products reveals different products produced by the two photocatalysts, providing evidence for the dominance of different degradation mechanisms for the two photocatalysts. In summary, this work suggests that BiOCl, potentially used in combination with TiO2, holds potential for degrading PPCPs in natural bodies of water.
Examining the Effect of Aromatic Substituents in Peptoid Catalysts of Stereoselective Trifluoromethylation Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2024-01-01
Creator: Daniel Chi
Access: Access restricted to the Bowdoin Community
Chromatin-conformation differences in natural populations of D. melanogaster Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2026-06-01
Date: 2021-01-01
Creator: Nicholas J. Purchase
Access: Access restricted to the Bowdoin Community
Functionality of Candida albicans She3 in the mRNA transport of Saccharomyces cerevisiae Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2021-01-01
Creator: Sheikh Omar Kunjo
Access: Access restricted to the Bowdoin Community
Investigating the Impacts of Drought on Turfgrass (Festuca arundinacea) Chlorophyll-a Fluorescence Emission
Date: 2024-01-01
Creator: Ayanna S Hatton
Access: Open access
- When photons from sunlight are absorbed by plants, they can take paths of photosynthesis, fluorescence, or energy dissipation. Instruments to quantify fluorescence have expanded in scale to allow measurements from satellites and flux towers using Solar-Induced Chlorophyll Fluorescence (SIF). Studies have found a positive correlation between SIF and gross primary productivity (GPP; representative of photosynthesis), suggesting SIF is a proxy for GPP. This conclusion encourages the use of SIF to inform decisions about carbon budgets and responding to climate change. Studies of fluorescence on the single-leaf scale have revealed that SIF measurements do not account for all variables nor is there an understanding of the impact of environmental factors, such as drought, on these measurements. In this project, tall fescue turfgrass was placed in one of four differing drought severities for 19 days. Leaf-level measurements of photosynthesis and pulse-amplitude modulated fluorescence were made, demonstrating stomatal closure and inhibition of photosynthesis. This physiological change caused greater photon allocation to energy dissipation. Changes in greenness and the utilization of photoprotective mechanisms such as senescence and anthocyanin accumulation were observed. This study has provided an understanding of the temporal, physiological, and visible impacts of drought on turfgrass to inform interpretations of SIF in future experiments. Caution is crucial in utilizing SIF as a proxy for GPP before further research into the impact of drought on SIF is completed.
Creating Enantioselective Peptoid Catalysts with 2-Picolylamine and 2-Picolinic Acid Catalytic Sites Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2024-01-01
Creator: Devin Kathleen O’Loughlin
Access: Access restricted to the Bowdoin Community
Physiological responses of the American lobster cardiovascular system to neuropeptide SGRNFLRFamide (SGRN)
Date: 2024-01-01
Creator: Andre Eden
Access: Open access
- During every second of a human’s life, the cardiovascular system is modulated by factors both intrinsic and extrinsic to the physiology of the heart. We can uncover new insights regarding the nature of our system through investigations of similar systems in other model species. One example materializes itself in the form of the American Lobster (Homarus americanus) whose single-chambered heart finds resemblance to the function and anatomy to that of humans. The lobster heart is powered by the cardiac ganglion (CG), a group of neurons that drive contractions of surrounding heart muscles, known as the myocardium. Both the CG and myocardium work in a feedback loop, with both intrinsic (afterload and preload) and extrinsic (temperature and neuropeptides) factors affecting cardiac output (CO) or the overall ability of the heart to carry out its primary function of nutrient distribution. In this paper, we examine how the addition of these factors into in vitro whole heart preparations affect CO and other associated variables. From experimentation, we conclude that the neuropeptide SGRNFLRFamide (SGRN) increases the heartbeat frequency and the active force exerted by the heart. We also conclude that increases in temperature decrease CO as higher temperatures decrease heartbeat frequency and the active force exerted by the heart. Lastly, we conclude that the effect of preload and afterload combined produce more robust effects on the CO and active force of the heart, potentially painting a better picture of what may happen in vivo.
Binding Energy Determination of CO2 Adsorption in MOF-74 with Diffusion Monte Carlo This record is embargoed.
- Embargo End Date: 2027-05-19
Date: 2022-01-01
Creator: Yucheng Hua
Access: Embargoed
Characterization of an Arabidopsis thaliana Adhesion Mutant This record is embargoed.
- Embargo End Date: 2025-05-14
Date: 2020-01-01
Creator: Jacob Dexter-Meldrum
Access: Embargoed