Showing 3541 - 3550 of 5713 Items
Date: 2020-12-01
Creator: Andrew E. Christie, Cindy D. Rivera, Catherine M. Call, Patsy S. Dickinson, Elizabeth A., Stemmler, J. Joe Hull
Access: Open access
- Over the past decade, in silico genome and transcriptome mining has led to the identification of many new crustacean peptide families, including the agatoxin-like peptides (ALPs), a group named for their structural similarity to agatoxin, a spider venom component. Here, analysis of publicly accessible transcriptomes was used to expand our understanding of crustacean ALPs. Specifically, transcriptome mining was used to investigate the phylogenetic/structural conservation, tissue localization, and putative functions of ALPs in decapod species. Transcripts encoding putative ALP precursors were identified from one or more members of the Penaeoidea (penaeid shrimp), Sergestoidea (sergestid shrimps), Caridea (caridean shrimp), Astacidea (clawed lobsters and freshwater crayfish), Achelata (spiny/slipper lobsters), and Brachyura (true crabs), suggesting a broad, and perhaps ubiquitous, conservation of ALPs in decapods. Comparison of the predicted mature structures of decapod ALPs revealed high levels of amino acid conservation, including eight identically conserved cysteine residues that presumably allow for the formation of four identically positioned disulfide bridges. All decapod ALPs are predicted to have amidated carboxyl-terminals. Two isoforms of ALP appear to be present in most decapod species, one 44 amino acids long and the other 42 amino acids in length, both likely generated by alternative splicing of a single gene. In carideans, a gene or terminal exon duplication appears to have occurred, with alternative splicing producing four ALPs, two 44 and two 42 amino acid isoforms. The identification of ALP precursor-encoding transcripts in nervous system-specific transcriptomes (e.g., Homarus americanus brain, eyestalk ganglia, and cardiac ganglion assemblies, finding confirmed using RT-PCR) suggests that members of this peptide family may serve as locally-released and/or hormonally-delivered neuromodulators in decapods. Their detection in testis- and hepatopancreas-specific transcriptomes suggests that members of the ALP family may also play roles in male reproduction and innate immunity/detoxification.
- Embargo End Date: 2025-05-16
Date: 2024-01-01
Creator: Leopold Felix Spieler
Access: Embargoed
Date: 2019-10-01
Creator: Patsy S. Dickinson, Heidi M. Samuel, Elizabeth A. Stemmler, Andrew E. Christie
Access: Open access
- The SIFamides are a broadly conserved arthropod peptide family characterized by the C-terminal motif –SIFamide. In decapod crustaceans, two isoforms of SIFamide are known, GYRKPPFNGSIFamide (Gly1-SIFamide), which is nearly ubiquitously conserved in the order, and VYRKPPFNGSIFamide (Val1-SIFamide), known only from members of the astacidean genus Homarus. While much work has focused on the identification of SIFamide isoforms in decapods, there are few direct demonstrations of physiological function for members of the peptide family in this taxon. Here, we assessed the effects of Gly1- and Val1-SIFamide on the cardiac neuromuscular system of two closely related species of Cancer crab, Cancer borealis and Cancer irroratus. In each species, both peptides were cardioactive, with identical, dose-dependent effects elicited by both isoforms in a given species. Threshold concentrations for bioactivity are in the range typically associated with hormonal delivery, i.e., 10−9 to 10−8 M. Interestingly, and quite surprisingly, while the predicted effects of SIFamide on cardiac output are similar in both C. borealis and C. irroratus, frequency effects predominate in C. borealis, while amplitude effects predominate in C. irroratus. These findings suggest that, while SIFamide is likely to increase cardiac output in both crabs, the mechanism through which this is achieved is different in the two species. Immunohistochemical/mass spectrometric data suggest that SIFamide is delivered to the heart hormonally rather than locally, with the source of hormonal release being midgut epithelial endocrine cells in both Cancer species. If so, midgut-derived SIFamide may function as a regulator of cardiac output during the process of digestion.
Date: 2007-05-15
Creator: Elizabeth A. Stemmler, Emily A. Bruns, Noah P. Gardner, Patsy S. Dickinson, Andrew E., Christie
Access: Open access
- In invertebrates, peptides possessing the carboxy (C)-terminal motif -RXRFamide have been proposed as the homologs of vertebrate neuropeptide Y (NPY). Using matrix assisted laser desorption/ionization mass spectrometry, in combination with sustained off-resonance irradiation collision-induced dissociation and chemical and enzymatic reactions, we have identified the peptide pEGFYSQRYamide from the neuroendocrine pericardial organ (PO) of the crab Pugettia producta. This peptide is likely the same as that previously reported, but misidentified, as PAFYSQRYamide in several earlier reports (e.g. [Li, L., Kelley, W.P., Billimoria, C.P., Christie, A.E., Pulver, S.R., Sweedler, J.V., Marder, E. 2003. Mass spectrometric investigation of the neuropeptide complement and release in the pericardial organs of the crab, Cancer borealis. J. Neurochem. 87, 642-656; Fu, Q., Kutz, K.K., Schmidt, J.J., Hsu, Y.W., Messinger, D.I., Cain, S.D., de la Iglesia, H.O., Christie, A.E., Li, L. 2005. Hormone complement of the Cancer productus sinus gland and pericardial organ: an anatomical and mass spectrometric investigation. J. Comp. Neurol. 493, 607-626.]). The -QRYamide motif contained in pEGFYSQRYamide is identical to that present in many vertebrate members of the NPY superfamily. Mass spectrometric analysis conducted on the POs of several other decapods showed that pEGFYSQRYamide is present in three other brachyurans (Cancer borealis, Cancer irroratus and Cancer productus) as well as in one species from another decapod infraorder (Lithodes maja, an anomuran). Thus, our findings show that at least some invertebrates possess NPY-like peptides in addition to those exhibiting an -RXRFamide C-terminus, and raise the question as to whether the invertebrate -QRYamides are functionally and/or evolutionarily related to the NPY superfamily. © 2007 Elsevier Inc. All rights reserved.
Date: 2009-03-05
Creator: Dale L Bumpers
Access: Open access
Biographial Note
Dale L. Bumpers was born on August 12, 1925, in Charleston, Arkansas. He attended the University of Arkansas, and during World War II he served in the U.S. Marine Corps. After being discharged, he attended Northwestern University Law School in Evanston, Illinois, where he received his law degree in 1951. He then returned to Charleston, Arkansas, where he began practicing law the following year. He ran for the state House in 1962 but lost. In 1970, he made a successful run for governor of Arkansas. He was elected to the U.S. States Senate in 1974, where he served until his retirement in 1999. During his tenure in the Senate, he never voted in favor of a constitutional amendment. He is married to Betty Flanagan Bumpers, who has spearheaded efforts to immunize children through the Dale and Betty Bumpers Vaccine Research Center.
Summary
Interview includes discussions of: coming into the Senate in the class of ’74; how George Mitchell rose to be majority leader: 1988 majority leader race; the role of money in politics and campaign finance reform; comparing leadership styles of Senators Byrd and Mitchell; Howard Baker as majority leader; Women Against Nuclear War / Peace Links and Betty Bumpers’ role in the organization, and the amendment Bumpers proposed to declare a national Peace Day; Robert Dole as majority leader; Tom Daschle as majority leader and as Mitchell’s protégé; George Mitchell’s inner circle; the evolution of partisanship in the Senate; the invasion of Iraq in 1991; the idea of constitutional amendments and Bumpers’s stance against them; the effects of living through the Depression and World War II on Bumpers’s generation; Mitchell’s decision to leave the Senate; Bumpers’s defense of Bill Clinton during the impeachment hearing; Bumpers’s presidential ambitions and choosing not to run in 1984; and the Bumpers’ involvement in desegregating the school in Charleston, Arkansas.
Date: 2009-02-07
Creator: Jeffrey 'Jeff' W Peterson
Access: Open access
Biographial Note
Jeffrey Ward Peterson was born on March 23, 1954, in Lexington, Massachusetts, to Jean H. and Dr. Merrill D. Peterson. He grew up in Lexington until age ten, then moved to Charlottesville, Virginia, in 1964. He attended Bowdoin College and studied away for a semester at American University, where he held an internship with the Senate Subcommittee on Intergovernmental Relations. After graduation he worked in Maine for two years, married, and moved to Seattle, Washington, to attend the University of Washington Graduate School of Public Affairs, where he earned a master’s degree while working part-time for the Environmental Protection Agency. He was later hired by the EPA to work at its Washington, DC, headquarters, and several years later he had the opportunity to work in Senator Mitchell’s office advising on water issues. He worked for the Environment and Public Works Committee for approximately eight years and then returned to work for the EPA. At the time of this interview, he was a senior policy advisor in the EPA Office of Water.
Summary
Interview includes discussion of: growing up in Charlottesville, Virginia; Bowdoin College; working for the Environmental Protection Agency in Seattle, Washington; being a McGovern delegate to the Virginia state convention in high school and how Mitchell staffers later gave him a hard time about it; Peterson’s connection with Maine; figuring out a way to get the EPA to have him work for Mitchell; working in Senator Mitchell’s personal office as an EPA consultant; Senators Chafee’s and Mitchell’s working relationship; working on Clean Water; Mitchell’s role in Clean Water hearings; the National Estuaries Program; working with the House staff; lengthy description of the Clean Water Act history; Mitchell’s role in maintaining ties between the Clean Water Act and Maine; the difficulty and benefits of reconciling Senate and House versions of a bill; conference process for the Clean Water Act; working for the Environment and Public Works Committee staff and the transition to that from working for the EPA in Mitchell’s office; Mitchell staff relationships; memo writing; the Clean Water reauthorizations; Peterson’s internship at the Senate Subcommittee on Intergovernmental Relations, chaired by Senator Muskie; Maine senators and the environment; other issues that Mitchell worked on; the issue of combined sewer overflows and the effect of Mitchell’s introducing legislation that addressed it; and Mitchell’s retirement from the Senate.
Date: 2009-04-03
Creator: Norman 'Norm' S Reef
Access: Open access
Biographial Note
Norman S. “Norm” Reef was born on August 16, 1933, in Portland, Maine, where he grew up with his parents, Samuel Reef and Dora Reef, and seven siblings. His father emigrated from Lithuania at age fourteen and worked as a cobbler. Norm grew up in the Jewish community of Portland, and the family struggled to make ends meet during the Depression and World War II. He attended Boston University and after two years joined the Army; the Korean War ended just as he completed training. He returned to study public relations at Boston University and then attended law school to help with his brothers’ insurance finance business. After being married, he set up a law practice in Portland. He became part of the fund-raising group for the Maine Democratic Party and encouraged Governor Brennan to appoint Mitchell to the Senate. He has also done legal work for Mitchell and is a personal friend. His daughter, Grace Reef, worked as a legislative assistant in Mitchell’s U.S. Senate office. He retired in 1989 from full-time practice of law. At the time of this interview, he was working on bringing about the production of a patent he holds for an industrial waste incinerator that generates power, associated with the firm Maine Microfurnace.
Summary
Interview includes discussion of: family and educational background; the Jewish community in Portland; growing up in a large family during WWII; the Army and being a dental technician in Texas; Boston University and being president of a fraternity; working with his brothers and going to law school; joining the finance committee for the Maine Democratic Party; Muskie’s swearing in as secretary of state 1980; meeting President Clinton; why Mitchell lost the 1974 gubernatorial race; a meeting in Augusta and Brennan’s decision to appoint Mitchell to the Senate; Mitchell at hockey games and his involvement with the Red Sox; Mitchell’s relationship to Muskie; Mitchell as U.S. attorney; humorous anecdotes about Grace’s internship in Mitchell’s office; Reef’s retirement and return to work; the Microfurnace patent; and Mitchell’s exceptional character.
Date: 2009-09-11
Creator: Carol Shapiro, Samuel 'Sam' Shapiro
Access: Open access
Biographial Note
Samuel Shapiro was born in Brownsville, Pennsylvania, on August 26, 1927. His parents were Maurice and Anna (Silver) Shapiro. His father was born in the Ukraine and his mother in Lithuania. He spent two years in the Navy, then attended the University of Pittsburg on the G.I. Bill, graduating in 1952. He married and moved to Waterville, Maine, in 1953 and served as Maine Democratic Party treasurer for thirteen years. He also ran several furniture stores with his father-in-law. He served as Maine state treasurer for 16 years (1980-1996) during the Brennan, McKernan, and King administrations. He worked with George Mitchell on the Democratic State Committee in the early 1960s; he was especially close to George Mitchell’s brother, Robbie, and they often played tennis. Carol Shapiro was born and raised in Waterville, Maine, and attended Colby College. She and George Mitchell were high school classmates.
Summary
Interview includes discussion of: family background; 1974 Maine gubernatorial campaign; Jim Longley story; military service; anti-Semitism; description of George Mitchell’s character and personality; state treasurers story; dating story; George Mitchell’s mother, Mary (Saad) Mitchell; Mitchell-Muskie comparison; Ken Curtis; and Bill Clinton story.
Date: 2010-01-22
Creator: John E Nale
Access: Open access
Biographial Note
John Elias Nale was born on September 4, 1949, in Farmerville, Louisiana, to Caroline Theresa (Boles) and Melvin Travis Nale. The family had moved to Louisiana from Bangor, Maine, returning first to Bangor and then to his mother’s home town, Waterville, Maine, during his childhood. He is a second cousin of George Mitchell; his grandmother and George Mitchell’s mother were sisters. Nale is a graduate of the University of Maine, Orono and the Franklin Pierce Law Center. Upon joining the bar, he practiced law in Waterville and Portland, Maine, including practicing law with his three brothers for fifteen years. At the time of this interview, he was practicing elder law at Nale Law Offices, Waterville, Maine.
Summary
Interview includes discussion of: growing up in Waterville, Maine, as next-door neighbor of the Mitchells; the Lebanese community in Waterville; Nale’s immigrant Lebanese ancestry; practicing elder law; Lebanese emigration to the United States; visiting Mitchell in Washington, DC, to celebrate Mitchell’s elevation to Senate majority leader; recollections of Mitchell’s mother, Mary (Saad) Mitchell.
Date: 2009-03-26
Creator: Grace Reef
Access: Open access
Biographial Note
Grace Reef grew up in Portland, Maine, with her father, Norman Reef, an attorney, and her mother, Patricia Reef. In 1974, as a twelve-year-old, she was the first female Little League baseball player, having sued to integrate girls into the program. She first heard of Senator Mitchell when he ran for governor in 1974. She attended Colby College, graduating in 1983 with a degree in public policy. During college she interned in Mitchell’s Senate office in Washington, D.C.; she worked as a legislative correspondent and was later promoted to be a legislative assistant, eventually becoming one of Mitchell’s senior advisors on children and poverty issues and economic development. She worked for minority leader Tom Daschle after Mitchell retired in 1994, continuing to work on issues of welfare reform and child care. She also worked for Senator Chris Dodd as the minority staff director of the Senate Committee on Health, Education, Labor, and Pensions Subcommittee on Children and Families. She has served as director of intergovernmental affairs for the Children’s Defense Fund. At the time of this interview, she was chief of policy and evaluation for the National Association of Child Care Resource and Referral Agencies (NACCRRA).
Summary
Interview includes discussion of: interning in Mitchell’s Senate office; the legislative process; tax reform in the mid-1980s; Mitchell’s decision-making process; the Family Support Act; doing research on child care providers in Maine; child care and development block grants; the Better Child Care Act; the structure of the Senate and the work Mitchell had to do to get legislation passed; Title 4A At Risk Child Care; the compromise on child care vouchers (church-state); Mitchell’s “divide and conquer” approach to dealing with different senators’ doubts and bringing them on board; the Family Medical Leave Act; anecdote of Reef’s presence in the Rose Garden with Mitchell when the Family Medical Leave Act was signed; George H.W. Bush’s saying that they needed a “kinder, gentler nation”; Environment and Public Works Committee work and highway funding; the formula for gas tax returns; miscalculating the formula and watching the bill on the floor of the Senate for three weeks; the National Affordable Housing Act; Mitchell and the Maine delegation; Mitchell’s patience as his greatest attribute; and Mitchell’s sense of humor.