Showing 3551 - 3560 of 5831 Items
Bowdoin College Catalogue (1961-1962)
Date: 1962-01-01
Access: Open access
- Bowdoin College Bulletin no. 342
Seasons Without Borders: the Ali Smith Quartet
Date: 2021-01-01
Creator: Claire M Burns
Access: Open access
- This project considers how the novels of contemporary Scottish author Ali Smith work to destabilize traditional constructions of temporal, formal, national, and gender borders. The motifs of the border and the border identity have been thematically pervasive in Scottish literary history, as reflected in the recurrence of the Scottish split identity. This thesis explores how the borders that have become essential to the construction of Scottish national literature, often relying on binaristic categorizations, have been disestablished in the contemporary era. Ali Smith’s novels, particularly the novels of her seasonal quartet, introduce forms and figures that highlight the instability of many of these borders, challenging fixed representations of the border identity. Through this focus on Scotland, Smith constructs a template for a consideration of national identity beyond the boundaries of Scotland, extending toward a more global sensibility.
AMGSEFLamide, a member of a broadly conserved peptide family, modulates multiple neural networks in Homarus americanus
Date: 2019-01-01
Creator: Patsy S. Dickinson, Evyn S. Dickinson, Emily R. Oleisky, Cindy D. Rivera, Meredith E., Stanhope, Elizabeth A. Stemmler, J. Joe Hull, Andrew E. Christie
Access: Open access
- Recent genomic/transcriptomic studies have identified a novel peptide family whose members share the carboxyl terminal sequence –GSEFLamide. However, the presence/identity of the predicted isoforms of this peptide group have yet to be confirmed biochemically, and no physiological function has yet been ascribed to any member of this peptide family. To determine the extent to which GSEFLamides are conserved within the Arthropoda, we searched publicly accessible databases for genomic/transcriptomic evidence of their presence. GSEFLamides appear to be highly conserved within the Arthropoda, with the possible exception of the Insecta, in which sequence evidence was limited to the more basal orders. One crustacean in which GSEFLamides have been predicted using transcriptomics is the lobster, Homarus americanus. Expression of the previously published transcriptome-derived sequences was confirmed by reverse transcription (RT)-PCR of brain and eyestalk ganglia cDNAs; mass spectral analyses confirmed the presence of all six of the predicted GSEFLamide isoforms – IGSEFLamide, MGSEFLamide, AMGSEFLamide, VMGSEFLamide, ALGSEFLamide and AVGSEFLamide – in H. americanus brain extracts. AMGSEFLamide, of which there are multiple copies in the cloned transcripts, was the most abundant isoform detected in the brain. Because the GSEFLamides are present in the lobster nervous system, we hypothesized that they might function as neuromodulators, as is common for neuropeptides. We thus asked whether AMGSEFLamide modulates the rhythmic outputs of the cardiac ganglion and the stomatogastric ganglion. Physiological recordings showed that AMGSEFLamide potently modulates the motor patterns produced by both ganglia, suggesting that the GSEFLamides may serve as important and conserved modulators of rhythmic motor activity in arthropods.
Characterization of the mature form of a β-defensin-like peptide, Hoa-D1, in the lobster Homarus americanus
Date: 2018-09-01
Creator: Giap H. Vu, Daniel Do, Cindy D. Rivera, Patsy S. Dickinson, Andrew E., Christie, Elizabeth A. Stemmler
Access: Open access
- We report on the characterization of the native form of an American lobster, Homarus americanus, β-defensin-like putative antimicrobial peptide, H. americanus defensin 1 (Hoa-D1), sequenced employing top-down and bottom-up peptidomic strategies using a sensitive, chip-based nanoLC-QTOF-MS/MS instrument. The sequence of Hoa-D1 was determined by mass spectrometry; it was found to contain three disulfide bonds and an amidated C-terminus. The sequence was further validated by searching publicly-accessible H. americanus expressed sequence tag (EST) and transcriptome shotgun assembly (TSA) datasets. Hoa-D1, SYVRScSSNGGDcVYRcYGNIINGAcSGSRVccRSGGGYamide (with c representing a cysteine participating in a disulfide bond), was shown to be related to β-defensin-like peptides previously reported from Panulirus japonicas and Panulirus argus. We found Hoa-D1 in H. americanus hemolymph, hemocytes, the supraoesophageal ganglion (brain), eyestalk ganglia, and pericardial organ extracts, as well as in the plasma of some hemolymph samples. Using discontinuous density gradient separations, we fractionatated hemocytes and localized Hoa-D1 to hemocyte sub-populations. While Hoa-D1 was detected in semigranulocytes and granulocytes using conventional proteomic strategies for analysis, the direct analysis of cell lysates exposed evidence of Hoa-D1 processing, including truncation of the C-terminal tyrosine residue, in the granulocytes, but not semigranulocytes. These measurements demonstrate the insights regarding post-translational modifications and peptide processing that can be revealed through the MS analysis of intact peptides. The identification of Hoa-D1 as a widely-distributed peptide in the lobster suggests the possibility that it may be pleiotropic, with functions in addition to its proposed role as an antimicrobial molecule in the innate immune system.
Brutal Encounters: Primitivity, Politics, and the Postmodern Revolution
Date: 2021-01-01
Creator: Archer Thomas
Access: Open access
- The switch from late modernism to postmodernism in Western aesthetic theory and criticism took place in the mid-to-late 20th century, radically changing the face of cultural criticism. Much has been written on how postmodernism broke from modernism, but what factors paved its way in the decades following the Second World War? This paper argues that postmodernism represents both a reaction to and a necessary evolution of late modernism, specifically as it manifests in architecture, politics, and the politics of architecture. It focuses on the crisis of confidence among Western left-wing circles following the upheaval of the Second World War and posits that, because of this upheaval, primitivism came to dominate the epistemology of a renewed modernism led by figures such as Clement Greenberg, Reyner Banham, and the practitioners of “the New Brutalism.” The paper then explores how the Western left-wing reaction to developments like decolonization and postwar modernization challenged primitivity’s newfound importance, resulting in a shift towards a “postmodern populism” in aesthetics and politics by the 1960s as described by Jean Baudrillard, Fredric Jameson, Robert Venturi, and Reyner Banham.
Comparison between the fCCZ4 and BSSN formulations of Einstein equations in spherical polar coordinates
Date: 2015-01-01
Creator: N. Sanchis-Gual, P.J. Montero, J.A. Font, E. Müller, T.W., Baumgarte
Access: Open access
Bowdoin College Catalogue (1968-1969)
Date: 1969-01-01
Access: Open access
- Bowdoin College Bulletin no. 370
Characterization of plexinA and two distinct semaphorin1a transcripts in the developing and adult cricket Gryllus bimaculatus
Date: 2020-03-01
Creator: Hadley W. Horch, Sara B. Spicer, Isabel I.C. Low, Colby T. Joncas, Eleanor D., Quenzer, Hikmah Okoya, Lisa M. Ledwidge, Harrison P. Fisher
Access: Open access
- Guidance cues act during development to guide growth cones to their proper targets in both the central and peripheral nervous systems. Experiments in many species indicate that guidance molecules also play important roles after development, though less is understood about their functions in the adult. The Semaphorin family of guidance cues, signaling through Plexin receptors, influences the development of both axons and dendrites in invertebrates. Semaphorin functions have been extensively explored in Drosophila melanogaster and some other Dipteran species, but little is known about their function in hemimetabolous insects. Here, we characterize sema1a and plexA in the cricket Gryllus bimaculatus. In fact, we found two distinct predicted Sema1a proteins in this species, Sema1a.1 and Sema1a.2, which shared only 48% identity at the amino acid level. We include a phylogenetic analysis that predicted that many other insect species, both holometabolous and hemimetabolous, express two Sema1a proteins as well. Finally, we used in situ hybridization to show that sema1a.1 and sema1a.2 expression patterns were spatially distinct in the embryo, and both roughly overlap with plexA. All three transcripts were also expressed in the adult brain, mainly in the mushroom bodies, though sema1a.2 was expressed most robustly. sema1a.2 was also expressed strongly in the adult thoracic ganglia while sema1a.1 was only weakly expressed and plexA was undetectable.