Showing 4361 - 4370 of 5709 Items
All Maine Biennial '79
Date: 1979-01-01
Access: Open access
- "Exhibition dates: July 27-September 16, 1979." "Supported by the Maine State Commission on the Arts and the Humanities."
Examination of tooth-specific cis-regulation of the dlx2b gene during zebrafish development This record is embargoed.
- Embargo End Date: 2025-05-14
Date: 2020-01-01
Creator: Yujin Moon
Access: Embargoed
Metabolic Inhibitors Induce Species-Specific Defects in Bacterial Glycosylation Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2020-01-01
Creator: Ilana R. Olin
Access: Access restricted to the Bowdoin Community
A Gift Of Knowing: The Art Of Dorothea Rockburne
Date: 2015-01-01
Access: Open access
- Published for the exhibition A Gift of Knowing: The Art of Dorothea Rockburne at the Bowdoin College Museum of Art from March 14 through April 26, 2015, supported by the Elizabeth B. G. Hamlin Fund and the Shapell Family Art Fund. Design by Wilcox Design, Cambridge, Massachusetts Copyright© 2015 Bowdoin College
Linking genotype to phenotype in a changing ocean: inferring the genomic architecture of a blue mussel stress response with genome-wide association
Date: 2018-03-01
Creator: S. E. Kingston, P. Martino, M. Melendy, F. A. Reed, D. B., Carlon
Access: Open access
- A key component to understanding the evolutionary response to a changing climate is linking underlying genetic variation to phenotypic variation in stress response. Here, we use a genome-wide association approach (GWAS) to understand the genetic architecture of calcification rates under simulated climate stress. We take advantage of the genomic gradient across the blue mussel hybrid zone (Mytilus edulis and Mytilus trossulus) in the Gulf of Maine (GOM) to link genetic variation with variance in calcification rates in response to simulated climate change. Falling calcium carbonate saturation states are predicted to negatively impact many marine organisms that build calcium carbonate shells – like blue mussels. We sampled wild mussels and measured net calcification phenotypes after exposing mussels to a ‘climate change’ common garden, where we raised temperature by 3°C, decreased pH by 0.2 units and limited food supply by filtering out planktonic particles >5 μm, compared to ambient GOM conditions in the summer. This climate change exposure greatly increased phenotypic variation in net calcification rates compared to ambient conditions. We then used regression models to link the phenotypic variation with over 170 000 single nucleotide polymorphism loci (SNPs) generated by genotype by sequencing to identify genomic locations associated with calcification phenotype, and estimate heritability and architecture of the trait. We identified at least one of potentially 2–10 genomic regions responsible for 30% of the phenotypic variation in calcification rates that are potential targets of natural selection by climate change. Our simulations suggest a power of 13.7% with our study's average effective sample size of 118 individuals and rare alleles, but a power of >90% when effective sample size is 900.
Multiple transcriptome mining coupled with tissue specific molecular cloning and mass spectrometry provide insights into agatoxin-like peptide conservation in decapod crustaceans
Date: 2020-12-01
Creator: Andrew E. Christie, Cindy D. Rivera, Catherine M. Call, Patsy S. Dickinson, Elizabeth A., Stemmler, J. Joe Hull
Access: Open access
- Over the past decade, in silico genome and transcriptome mining has led to the identification of many new crustacean peptide families, including the agatoxin-like peptides (ALPs), a group named for their structural similarity to agatoxin, a spider venom component. Here, analysis of publicly accessible transcriptomes was used to expand our understanding of crustacean ALPs. Specifically, transcriptome mining was used to investigate the phylogenetic/structural conservation, tissue localization, and putative functions of ALPs in decapod species. Transcripts encoding putative ALP precursors were identified from one or more members of the Penaeoidea (penaeid shrimp), Sergestoidea (sergestid shrimps), Caridea (caridean shrimp), Astacidea (clawed lobsters and freshwater crayfish), Achelata (spiny/slipper lobsters), and Brachyura (true crabs), suggesting a broad, and perhaps ubiquitous, conservation of ALPs in decapods. Comparison of the predicted mature structures of decapod ALPs revealed high levels of amino acid conservation, including eight identically conserved cysteine residues that presumably allow for the formation of four identically positioned disulfide bridges. All decapod ALPs are predicted to have amidated carboxyl-terminals. Two isoforms of ALP appear to be present in most decapod species, one 44 amino acids long and the other 42 amino acids in length, both likely generated by alternative splicing of a single gene. In carideans, a gene or terminal exon duplication appears to have occurred, with alternative splicing producing four ALPs, two 44 and two 42 amino acid isoforms. The identification of ALP precursor-encoding transcripts in nervous system-specific transcriptomes (e.g., Homarus americanus brain, eyestalk ganglia, and cardiac ganglion assemblies, finding confirmed using RT-PCR) suggests that members of this peptide family may serve as locally-released and/or hormonally-delivered neuromodulators in decapods. Their detection in testis- and hepatopancreas-specific transcriptomes suggests that members of the ALP family may also play roles in male reproduction and innate immunity/detoxification.
In silico analyses suggest the cardiac ganglion of the lobster, Homarus americanus, contains a diverse array of putative innexin/innexin-like proteins, including both known and novel members of this protein family
Date: 2020-06-01
Creator: Andrew E. Christie, J. Joe Hull, Patsy S. Dickinson
Access: Open access
- Gap junctions are physical channels that connect adjacent cells, permitting the flow of small molecules/ions between the cytoplasms of the coupled units. Innexin/innexin-like proteins are responsible for the formation of invertebrate gap junctions. Within the nervous system, gap junctions often function as electrical synapses, providing a means for coordinating activity among electrically coupled neurons. While some gap junctions allow the bidirectional flow of small molecules/ions between coupled cells, others permit flow in one direction only or preferentially. The complement of innexins present in a gap junction determines its specific properties. Thus, understanding innexin diversity is key for understanding the full potential of electrical coupling in a species/system. The decapod crustacean cardiac ganglion (CG), which controls cardiac muscle contractions, is a simple pattern-generating neural network with extensive electrical coupling among its circuit elements. In the lobster, Homarus americanus, prior work suggested that the adult neuronal innexin complement consists of six innexins (Homam-Inx1-4 and Homam-Inx6-7). Here, using a H. americanus CG-specific transcriptome, we explored innexin complement in this portion of the lobster nervous system. With the exception of Homam-Inx4, all of the previously described innexins appear to be expressed in the H. americanus CG. In addition, transcripts encoding seven novel putative innexins (Homam-Inx8-14) were identified, four (Homam-Inx8-11) having multiple splice variants, e.g., six for Homam-Inx8. Collectively, these data indicate that the innexin complement of the lobster nervous system in general, and the CG specifically, is likely significantly greater than previously reported, suggesting the possibility of expanded gap junction diversity and function in H. americanus.
Chambers of Reflection: Rousseau, Tocqueville, and Self-Government in the Digital Age
Date: 2020-01-01
Creator: John Sweeney
Access: Open access
- Jean-Jacques Rousseau and Alexis de Tocqueville each warn that the dominant cultures of their days may hinder the project of self-government. Against the backdrop of advancing Enlightenment philosophy, Rousseau writes that as social visibility increases relative to intimate connection, the drive for recognition corrupts self-love. Following the American and French revolutions, Tocqueville explores the democratic erosion of social hierarchies. He writes that a rise in individualism may obscure “self-interest well-understood”—the perspective gained through collaboration with others, thoughtful reflection, and reverence for truths that lie beyond the dictates of cursory instincts. In this project, I apply these political theories to the Digital Age. I explain how the distinction between the physical world and the digital realm has actualized Rousseau’s depiction of double men, “always appearing to relate everything to others and never relating anything except to themselves alone.” In the era of social distancing, technological evolution threatens to induce regression in the sociability and reflective agency that promote our capacity for self-government. Accordingly, I argue that Rousseau’s theory of corrupted drive for recognition and Tocqueville’s theory of individualism inform a new danger to political freedom: digital tribalism.
From Shadow to Spotlight: Minoritarian Characters, Representative Failures, and High School Powerarchy in Teen Television This record is embargoed.
- Embargo End Date: 2028-05-18
Date: 2023-01-01
Creator: Paloma Ada Aguirre
Access: Embargoed
Fact vs. Faction: Polarization in the Information Age
Date: 2016-05-01
Creator: Noah Finberg
Access: Open access
- How can individuals in the contemporary media and political environment form better political beliefs? In chapter one, this thesis considers what it means to say American politics is polarized. It evaluates the extent of polarization in American politics. And it presents original evidence that suggests that just as the public and members of Congress have polarized, so too has American political discourse. Through the lens of political psychology, chapter two investigates how America’s polarized politics has influenced the quality of individuals’ beliefs. Chapter three explores the role that the media plays in encouraging or minimizing the biased information processing practices identified in chapter two. Finally, I conclude by arguing that individuals need to fundamentally rethink how they consume political information; advocate for the creation of a completely new social media platform specifically designed to encourage political deliberation; and outline what such a platform might look like.