Showing 5611 - 5620 of 5713 Items
Date: 2024-01-25
Creator: Heather Bruce, Hadley Wilson Horch
Access: Open access
- Visualizing the expression of genes is a fundamental tool in molecular biology. Traditional colorimetric in situ hybridization using long RNA probes has been a staple for visualizing gene expression but has many drawbacks. In situ HCR v3.0, developed by Choi et. al. 2018, offers improvements over traditional in situs in nearly every aspect: probes can simply be ordered rather than painstakingly cloned and transcribed, which also makes them cost-effective; an HCR takes just three days to complete rather than five or more days; HCR is robust and works well for first-time users; and HCR probes can be multiplexed, allowing four to eight genes to be visualized in a single sample. HCR has been used successfully in many arthropods, including insects (Drosophila, Tribolium), crustaceans (Parhyale, Daphnia, Artemia), and chelicerates (Limulus horseshoe crab, Acanthoscurria tarantula). In this demo, you will learn how to design and order HCR probes as well as best practices for experimental design.
- Restriction End Date: 2026-06-01
Date: 2021-01-01
Creator: Kodie R Garza
Access: Access restricted to the Bowdoin Community
- Embargo End Date: 2026-05-20
Date: 2021-01-01
Creator: Lianna Harrington
Access: Embargoed
Date: 2015-09-01
Creator: Patsy S. Dickinson, Anirudh Sreekrishnan, Molly A. Kwiatkowski, Andrew E. Christie
Access: Open access
- Although the crustacean heart is modulated by a large number of peptides and amines, few of these molecules have been localized to the cardiac ganglion itself; most appear to reach the cardiac ganglion only by hormonal routes. Immunohistochemistry in the American lobster Homarus americanus indicates that pyrokinins are present not only in neuroendocrine organs ( pericardial organ and sinus gland), but also in the cardiac ganglion itself, where pyrokinin-positive terminals were found in the pacemaker cell region, as well as surrounding the motor neurons. Surprisingly, the single pyrokinin peptide identified from H. americanus, FSPRLamide, which consists solely of the conserved FXPRLamide residues that characterize pyrokinins, did not alter the activity of the cardiac neuromuscular system. However, a pyrokinin from the shrimp Litopenaeus vannamei [ADFAFNPRLamide, also known as Penaeus vannamei pyrokinin 2 (PevPK2)] increased both the frequency and amplitude of heart contractions when perfused through the isolated whole heart. None of the other crustacean pyrokinins tested (another from L. vannamei and two from the crab Cancer borealis) had any effect on the lobster heart. Similarly, altering the PevPK2 sequence either by truncation or by the substitution of single amino acids resulted in much lower or no activity in all cases; only the conservative substitution of serine for alanine at position 1 resulted in any activity on the heart. Thus, in contrast to other systems (cockroach and crab) in which all tested pyrokinins elicit similar bioactivities, activation of the pyrokinin receptor in the lobster heart appears to be highly isoform specific.
Date: 1992-01-01
Creator: D. Bortoletto, D. N. Brown, J. Dominick, R. L. McIlwain, D. H., Miller, M. Modesitt, E. I. Shibata, S. Schaffner, I. P.J. Shipsey, M. Battle, H. Kroha, K. Sparks, E. H. Thorndike, C. H. Wang, M. Goldberg, T. Haupt, N. Horwitz, V. Jain, G. C. Moneti, Y. Rozen, P. Rubin, T. Skwarnicki, V. Sharma, S. Stone, M. Thusalidas, W. M. Yao, G. Zhu, A. V. Barnes, J. Bartelt, S. E. Csorna, T. Letson
Access: Open access
- We have studied hadronic decays of B mesons. We report measurements of exclusive branching ratios of several charm decay modes of B mesons to final states with a D or D* and one to three charged pions or a charged and to final states with a or , a kaon, and up to two charged pions. We have also measured inclusive branching ratios for B decays to D and D* and the spectra of these particles in B decays. The total charm content in B decay is found to be (10112)%. The branching ratios and spectra are compared to form-factor models. We extract the parameters a1 and a2 of the model of Bauer, Stech, and Wirbel and the DS decay constant. The masses of the B0 and B- mesons are measured. The mass difference between B0 and B- is found to be -0.40.60.5 MeV/c2. © 1992 The American Physical Society.
Date: 1992-01-01
Creator: D. S. Akerib, B. Barish, M. Chadha, D. F. Cowen, G., Eigen, J. S. Miller, J. Urheim, A. J. Weinstein, D. Acosta, G. Masek, B. Ong, H. Paar, M. Sivertz, A. Bean, J. Gronberg, R. Kutschke, S. Menary, R. J. Morrison, H. N. Nelson, J. D. Richman, H. Tajima, D. Schmidt, D. Sperka, M. S. Witherell, M. Procario, S. Yang, M. Daoudi, W. T. Ford, D. R. Johnson, K. Lingel, M. Lohner
Access: Open access
- The tau lepton electron branching fraction has been measured with the CLEO II detector at the Cornell Electron Storage Ring as Be=0.17490.00140.0022, with the first error statistical and the second systematic. The measurement involves counting electron-positron annihilation events in which both taus decay to electrons, and normalizing to the number of tau-pair decays expected from the measured luminosity. Detected photons in these events constitute a definitive observation of tau decay radiation. © 1992 The American Physical Society.
Date: 2015-12-01
Creator: Andrew E. Christie, Megan Chi, Tess J. Lameyer, Micah G. Pascual, Devlin N., Shea, Meredith E. Stanhope, David J. Schulz, Patsy S. Dickinson
Access: Open access
- Peptides are the largest and most diverse class of molecules used for neurochemical communication, playing key roles in the control of essentially all aspects of physiology and behavior. The American lobster, Homarus americanus, is a crustacean of commercial and biomedical importance; lobster growth and reproduction are under neuropeptidergic control, and portions of the lobster nervous system serve as models for understanding the general principles underlying rhythmic motor behavior (including peptidergic neuromodulation). While a number of neuropeptides have been identified from H. americanus, and the effects of some have been investigated at the cellular/systems levels, little is currently known about the molecular components of neuropeptidergic signaling in the lobster. Here, a H. americanus neural transcriptome was generated and mined for sequences encoding putative peptide precursors and receptors; 35 precursor- and 41 receptor-encoding transcripts were identified. We predicted 194 distinct neuropeptides from the deduced precursor proteins, including members of the adipokinetic hormone-corazonin-like peptide, allatostatin A, allatostatin C, bursicon, CCHamide, corazonin, crustacean cardioactive peptide, crustacean hyperglycemic hormone (CHH), CHH precursor-related peptide, diuretic hormone 31, diuretic hormone 44, eclosion hormone, FLRFamide, GSEFLamide, insulin-like peptide, intocin, leucokinin, myosuppressin, neuroparsin, neuropeptide F, orcokinin, pigment dispersing hormone, proctolin, pyrokinin, SIFamide, sulfakinin and tachykinin-related peptide families. While some of the predicted peptides are known H. americanus isoforms, most are novel identifications, more than doubling the extant lobster neuropeptidome. The deduced receptor proteins are the first descriptions of H. americanus neuropeptide receptors, and include ones for most of the peptide groups mentioned earlier, as well as those for ecdysistriggering hormone, red pigment concentrating hormone and short neuropeptide F. Multiple receptors were identified for most peptide families. These data represent the most complete description of the molecular underpinnings of peptidergic signaling in H. americanus, and will serve as a foundation for future gene-based studies of neuropeptidergic control in the lobster.
Date: 1991-01-01
Creator: Y. Kubota, J. K. Nelson, D. Perticone, R. Poling, S., Schrenk, G. Crawford, R. Fulton, T. Jensen, D. R. Johnson, H. Kagan, R. Kass, R. Malchow, F. Morrow, J. Whitmore, P. Wilson, D. Bortoletto, D. Brown, J. Dominick, R. L. McIlwain, D. H. Miller, M. Modesitt, C. R. Ng, S. F. Schaffner, E. I. Shibata, I. P.J. Shipsey, M. Battle, H. Kroha, K. Sparks, E. H. Thorndike, C. H. Wang, M. S. Alam
Access: Open access
- The spin alignment of D*+ mesons produced in e+e- annihilation at s=10.5 GeV is obtained from a study of the angular distribution of the decay D*+D0+. The alignment is studied as a function of momentum and compared to theoretical predictions. We find an average value of the spin alignment parameter of =0.040.020.01. We obtain a model-dependent measurement of the probability of producing a vector particle PV=0.770.020.01 for D mesons. © 1991 The American Physical Society.
Date: 1990-01-01
Creator: P. Avery, D. Besson, L. Garren, J. Yelton, K., Kinoshita, F. M. Pipkin, M. Procario, Richard Wilson, J. Wolinski, D. Xiao, Y. Zhu, R. Ammar, P. Baringer, D. Coppage, R. Davis, P. Haas, M. Kelly, N. Kwak, Ha Lam, S. Ro, Y. Kubota, J. K. Nelson, D. Perticone, R. Poling, R. Fulton, T. Jensen, D. R. Johnson, H. Kagan, R. Kass, F. Morrow, J. Whitmore
Access: Open access
- We report a measurement of polarization in the two-body decay c+, in nonresonant e+e- interactions from data taken with the CLEO detector. Using these data we have determined the parity-violating asymmetry decay parameter c to be -1.0-0.0+0.4. We see no evidence for significant c+ polarization. © 1990 The American Physical Society.