Showing 61 - 67 of 67 Items
The Role of ELMO5 in Arabidopsis thaliana Cell Adhesion
Date: 2022-01-01
Creator: Isabel Kristina Ball
Access: Open access
- Plant cell growth and development relies on proper cellular adhesion. As the extracellular matrix serves as the area of connection between two cells, its synthesis and maintenance are essential for cellular adhesion. The middle lamella region, the layer of the extracellular matrix between two adjacent cell walls, is diffuse with the polysaccharide pectin due to its delivery by Golgi vesicles early during cell division. A Ruthenium Red screen for cellular adhesion mutants identified the family of 5 ELMO proteins that are critical for proper cellular adhesion. To further our understanding of plant cellular adhesion and pathways of pectin synthesis and modification, this work investigates ELMO5. Plants homozygous for a T-DNA insertion in ELMO5 and a new deletion mutant allele generated using CRSPR do not have a cellular adhesion phenotype, suggesting it is either not critical for cellular adhesion or is redundant with another gene. Redundancy within the ELMO family is identified through the analysis of double mutants of elmo5 and each of the other four elmo genes. Both elmo1-/- elmo5-/-and elmo4-/- elmo5-/-mutants have a visibly worse cellular adhesion defect phenotype, suggesting partial redundancy through the ELMO family. The mutants are also rescued by growth on agar, pointing to the importance of turgor pressure and osmotic potential in modulating cellular adhesion. Both ELMO4 and ELMO5 were found to localize to the Golgi using a GFP fusion, consistent with a role for ELMOs as scaffold for pectin biosynthesis.
Characterization of Spaetzle-Toll Ligand-Receptor Pairs in Gryllus bimaculatus Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2028-06-01
Date: 2023-01-01
Creator: Tabarak Al Musawi
Access: Access restricted to the Bowdoin Community
The Role of Pectin Methyl Esterase in Pectin Activation of WAK Regulated Stress Response in Arabidopsis thaliana Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2014-05-01
Creator: Nicholas J Saba
Access: Access restricted to the Bowdoin Community
CRISPR Mutagenesis of the WAK Locus Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2016-01-01
Creator: Cody P Woods
Access: Access restricted to the Bowdoin Community
Characterizing variation in enhancer usage within and between natural populations of Drosophila by comparing chromatin conformation in non-coding DNA This record is embargoed.
- Embargo End Date: 2027-05-19
Date: 2022-01-01
Creator: Serena Jonas
Access: Embargoed
Activation of Hydrogen by Sterically Modulated Coinage Metal Catalysts via Mutual Quenching of Hard/Soft Acid/Base Mismatches
Date: 2024-01-01
Creator: Zach Leibowitz
Access: Open access
- To mitigate the devastating environmental impacts of climate change in the coming decades, it is imperative that we replace the use of fossil fuels with renewable energy sources such as wind, solar, and hydroelectric. As these renewable energy sources are inherently intermittent, there exists a need for sustainable mechanisms to store renewable energy for later use. While the direct use of dihydrogen (H2) as a combustible fuel would allow for energy storage without the harmful release of carbon dioxide (CO2) upon combustion, the practicality of H2 as a synthetic fuel is limited by its low volumetric energy density. Combining sustainable H2 production (e.g. electrolysis using energy from renewable sources) with subsequent carbon fixation (e.g. the hydrogenation of CO2) represents a promising pathway to the sustainable production of high-density synthetic fuels. We hypothesize that such a process could be catalyzed by an IPr**-supported catalyst containing a hard/soft acid/base (HSAB) mismatch, with a polarizable coinage metal acting as a soft acid. As such, the aim of our project is the construction of a catalogue of IPr**-supported copper, silver, and gold catalysts that we anticipate will facilitate the heterolysis of dihydrogen and subsequent hydrogenation of CO2. In the present paper, we report the synthesis and characterization of an IPr**-silver complex which will serve as a precursor to many of our proposed HSAB mismatch catalysts and discuss next steps as we construct our catalogue of catalysts.
Using Fluorogenic Monosaccharides to Detect and Identify Glycan-Degrading Enzymes Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2025-01-01
Creator: Esteban Tarazona Guzman
Access: Access restricted to the Bowdoin Community