Showing 1 - 6 of 6 Items
Enhancer usage variation assessed via chromatin-conformation within and among three species of Drosophila This record is embargoed.
- Embargo End Date: 2027-05-19
Date: 2022-01-01
Creator: Maia B. Granoski
Access: Embargoed
Investigating the role of eyes absent in photoreceptor axon targeting in Drosophila melanogaster
Date: 2021-01-01
Creator: Bethany J. Thach
Access: Open access
- The eya gene is essential to development of the Drosophila visual system and eye-specific loss of function mutations in the gene commonly result in the missing eye phenotype. The eya2m35g mutation carries a deletion of exon 1B and adjacent regulatory sequences. Flies carrying the eya2m35g allele exhibit a photoreceptor axon phenotype that has not previously been associated with the eya gene. To determine a potential role for eya in photoreceptor axon targeting, I characterized various phenotypes of eya2m35g mutants and generated additional eya alleles consisting of smaller deletions within the eya2m35g mutation to locate the genetic source of axonal disruption. Using immunofluorescence staining to visualize Eya protein, I found a loss of eya expression in the optic lobe region of eya2m35g stage 9 embryos and third instar larvae. I also observed a loss of retinal basal glial (RBG) cells in the larval eye disc. Finally, I demonstrated that the disconnected axon phenotype is generated when a region of the intron immediately downstream of exon 1B is deleted. These findings suggest that a possible regulatory element for eya that is essential for photoreceptor axon targeting exists in this intronic region.
Live imaging of somatic homolog pairing in Drosophila supports a button-based mechanism for pairing facilitated by the genetic insulator gypsy This record is embargoed.
- Embargo End Date: 2026-05-18
Date: 2023-01-01
Creator: Holden D. Hadfield
Access: Embargoed
Characterizing variation in enhancer usage within and between natural populations of Drosophila by comparing chromatin conformation in non-coding DNA This record is embargoed.
- Embargo End Date: 2027-05-19
Date: 2022-01-01
Creator: Serena Jonas
Access: Embargoed
Regulation of eyes absent gene expression in Drosophila melanogaster by Polycomb Group Response Elements Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2025-06-01
Date: 2020-01-01
Creator: Boris S. Dimitrov
Access: Access restricted to the Bowdoin Community
Comparing natural variation in enhancer usage within and among Drosophila species This record is embargoed.
- Embargo End Date: 2027-05-19
Date: 2022-01-01
Creator: Justin K. Yang
Access: Embargoed