Showing 1 - 6 of 6 Items
Wnt Signaling is Dispensable to Formation of the First Tooth in D. Rerio This record is embargoed.
- Embargo End Date: 2025-05-14
Date: 2020-01-01
Creator: Zachary C. LeBlanc
Access: Embargoed
Investigating enhancer regulation through chromatin conformation in Drosophila Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2020-01-01
Creator: Hannah D. Konkel
Access: Access restricted to the Bowdoin Community
Characterizing variation in enhancer usage within and between natural populations of Drosophila by comparing chromatin conformation in non-coding DNA This record is embargoed.
- Embargo End Date: 2027-05-19
Date: 2022-01-01
Creator: Serena Jonas
Access: Embargoed
Lake water chemistry and local adaptation shape NaCl toxicity in Daphnia ambigua
Date: 2024-01-01
Creator: Mary Alta Rogalski, Elizabeth S Baker, Clara M Benadon
Access: Open access
- Increasing application of road deicing agents (e.g., NaCl) has caused widespread salinization of freshwater environments. Chronic exposure to toxic NaCl levels can impact freshwater biota at genome to ecosystem scales, yet the degree of harm caused by road salt pollution is likely to vary among habitats and populations. The background ion chemistry of freshwater environments may strongly impact NaCl toxicity, with greater harm occurring in ion-poor, soft water conditions. In addition, populations exposed to salinization may evolve increased NaCl tolerance. Notably, if organisms are adapted to their natal lake water chemistry, toxicity responses may also vary among populations in a given test medium. We examined how this evolutionary and environmental context may interact in shaping NaCl toxicity with a pair of laboratory reciprocal transplant toxicity experiments, using natural populations of the water flea Daphnia ambigua from three lakes differing in ion availability. The lake water environment strongly influenced NaCl toxicity in both trials. NaCl greatly reduced reproduction and r in lake water from a low-ion/ calcium-poor environment compared with water from both a calcium-rich lake and an ion-rich coastal lake. Daphnia from this coastal lake were most robust to the effects of NaCl. A significant population x environment interaction shaped survival in both trials, suggesting that local adaptation to the test waters used contributed to toxicity responses. Our findings that the lake water environment, adaptation to that environment, and adaptation to a focal contaminant may shape toxicity demonstrate the importance of considering environmental and biological complexity in mitigating pollution impacts.
Injecting gryllus bimaculatus eggs
Date: 2019-08-01
Creator: Samantha K. Barry, Taro Nakamura, Yuji Matsuoka, Christoph Straub, Hadley W., Horch, Cassandra G. Extavour
Access: Open access
- Altering gene function in a developing organism is central to different kinds of experiments. While tremendously powerful genetic tools have been developed in traditional model systems, it is difficult to manipulate genes or messenger RNA (mRNA) in most other organisms. At the same time, evolutionary and comparative approaches rely on an exploration of gene function in many different species, necessitating the development and adaptation of techniques for manipulating expression outside currently genetically tractable species. This protocol describes a method for injecting reagents into cricket eggs to assay the effects of a given manipulation on embryonic or larval development. Instructions for how to collect and inject eggs with beveled needles are described. This relatively straightforward technique is flexible and potentially adaptable to other insects. One can gather and inject dozens of eggs in a single experiment, and survival rates for buffer-only injections improve with practice and can be as high as 80%. This technique will support several types of experimental approaches including injection of pharmacological agents, in vitro capped mRNA to express genes of interest, double-stranded RNA (dsRNA) to achieve RNA interference, use of clustered regularly interspaced short palindromic repeats (CRISPR) in concert with CRISPR-associated protein 9 (Cas9) reagents for genomic modification, and transposable elements to generate transient or stable transgenic lines.
The identification and visualization of candidate early embryonic patterning genes in Bradysia coprophila This record is embargoed.
- Embargo End Date: 2027-05-16
Date: 2024-01-01
Creator: Sarah Conant
Access: Embargoed