Showing 1 - 10 of 14 Items
Cell Adhesion in Arabidopsis thaliana Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2019-05-01
Creator: Natasha Ann Belsky
Access: Access restricted to the Bowdoin Community
Genetic Analysis of Adhesion Protein ELMO3 in Arabidopsis thaliana
Date: 2022-01-01
Creator: Garrison Asper
Access: Open access
- The Extracellular Matrix (ECM) between plant cells is vital for structure, development, and intercellular adhesion. A pectin rich layer in between cells, the middle lamella, is largely responsible for regulating the adhesive properties of adjacent plant cells. Homogalacturonan (HG) pectin, the most common, is synthesized in the Golgi and secreted into the ECM where it undergoes calcium crosslinking, increasing its adhesive properties. Mutations in proteins essential for HG synthesis can reveal a severe adhesion defective phenotype, where the hypocotyls of dark grown Arabidopsis exhibit cell sloughing, curling, and general disorganization. A family of five ELMO proteins are suspected to act as scaffolds for pectin biosynthesis enzymes. ELMO1 and ELMO4 mutants exhibit an adhesion deficient phenotype, and a double mutant provides evidence of redundancy in function between ELMO1 and ELMO2. ELMO1-GFP co-immunoprecipitated with enzymes required for HG synthesis indicating its role as a scaffold protein. Double mutants of the other ELMO homologues were created to determine if they exhibit functional redundancy, and ELMO1 and ELMO3 appear partially redundant. A gene deletion of ELMO3 was also created using the CRSPR/Cas9 system, resulting in two distinct elmo3 deletion alleles, which were phenotypically identical to the original elmo3-/- mutant. All adhesion defective phenotypes can be partially suppressed by altering the osmoticum and hence turgor that provides pressure on adhesive cells. Lastly, ELMO3-GFP was localized to the Golgi, the site of pectin biosynthesis, further supporting a common role of the ELMOs in pectin biosynthesis.
Role of Polycomb group proteins in regulation of eyes absent gene expression in Drosophila melanogaster This record is embargoed.
- Embargo End Date: 2027-05-16
Date: 2024-01-01
Creator: Joanne Du
Access: Embargoed
Regulation of eyes absent gene expression in Drosophila melanogaster by Polycomb Group Response Elements Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2025-06-01
Date: 2020-01-01
Creator: Boris S. Dimitrov
Access: Access restricted to the Bowdoin Community
Radiation-induced changes in gene expression in Sciara coprophila Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2026-06-01
Date: 2021-01-01
Creator: Kodie R Garza
Access: Access restricted to the Bowdoin Community
Promoter Choice in Transvection at the eya Gene of Drosophila melanogaster This record is embargoed.
- Embargo End Date: 2027-05-15
Date: 2024-01-01
Creator: Victoria Dunphy
Access: Embargoed
Genetic Analysis of Cellular Adhesion in Arabidopsis thaliana
Date: 2021-01-01
Creator: Andrew Close Bolender
Access: Open access
- Plant cell adhesion is mediated by the extracellular matrix (ECM) or cell wall and plays an important role in plant morphogenesis and development. The amount, modification, and cleavage of pectin in the cell wall are major contributors to the adhesive properties of the ECM. To gain a more complete picture of plant cell adhesion processes, Arabidopsis thaliana seedlings were previously mutagenized and screened for hypocotyl adhesion defects. Genomic sequencing of one plant exhibiting an adhesion defect, isolate 242, showed that two mutations, one in cellulose synthase (CesA1) and another in a sugar transporter, are candidates for the causative mutation. This thesis reports that CesA1 is necessary for proper plant cell adhesion, while the sugar transporter encoded at At4g32390 is not. Dark grown seedlings homozygous for mutations in CesA1 stain in ruthenium red, indicating atypical adhesion, while those homozygous for null mutations in At4g32390 do not. Previous study of another adhesion mutant revealed ELMO1, a Golgi protein necessary for plant cell adhesion, and four additional homologs ELMO2-5 in the A. thaliana genome. Two of these homologs, ELMO2 and ELMO3, fused to GFP, colocalized with mCherry-MEM1 markers in the Golgi, but not mCherry-NLM12 ER markers, indicating that ELMO2 and ELMO3 are also Golgi proteins.
The Role of Pectin Methyl Esterase in Pectin Activation of WAK Regulated Stress Response in Arabidopsis thaliana Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2014-05-01
Creator: Nicholas J Saba
Access: Access restricted to the Bowdoin Community
Characterizing variation in enhancer usage within and between natural populations of Drosophila by comparing chromatin conformation in non-coding DNA This record is embargoed.
- Embargo End Date: 2027-05-19
Date: 2022-01-01
Creator: Serena Jonas
Access: Embargoed
Investigating the Role of Heterochromatin Protein 1 (HP1) in the Formation of Chromosome Compartments Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2025-06-01
Date: 2020-01-01
Creator: Diego Andres Villamarin
Access: Access restricted to the Bowdoin Community