Showing 1 - 2 of 2 Items

Date: 2009-12-15

Creator: J. S. Stevens, C. R. Cashman, C. M. Smith, K. M. Beale, D. W., Towle, A. E. Christie, P. S. Dickinson

Access: Open access

pQDLDHVFLRFamide is a highly conserved crustacean neuropeptide with a structure that places it within the myosuppressin subfamily of the FMRFamide-like peptides. Despite its apparent ubiquitous conservation in decapod crustaceans, the paracrine and/or endocrine roles played by pQDLDHVFLRFamide remain largely unknown. We have examined the actions of this peptide on the cardiac neuromuscular system of the American lobster Homarus americanus using four preparations: the intact animal, the heart in vitro, the isolated cardiac ganglion (CG), and a stimulated heart muscle preparation. In the intact animal, injection of myosuppressin caused a decrease in heartbeat frequency. Perfusion of the in vitro heart with pQDLDHVFLRFamide elicited a decrease in the frequency and an increase in the amplitude of heart contractions. In the isolated CG, myosuppressin induced a hyperpolarization of the resting membrane potential of cardiac motor neurons and a decrease in the cycle frequency of their bursting. In the stimulated heart muscle preparation, pQDLDHVFLRFamide increased the amplitude of the induced contractions, suggesting that myosuppressin modulates not only the CG, but also peripheral sites. For at least the in vitro heart and the isolated CG, the effects of myosuppressin were dose-dependent (10 -9 to 10-6mol l-1 tested), with threshold concentrations (10-8-10-7 mol l-1) consistent with the peptide serving as a circulating hormone. Although cycle frequency, a parameter directly determined by the CG, consistently decreased when pQDLDHVFLRFamide was applied to all preparation types, the magnitudes of this decrease differed, suggesting the possibility that, because myosuppressin modulates the CG and the periphery, it also alters peripheral feedback to the CG.

Date: 2024-01-01

Creator: Andre Eden

Access: Open access

During every second of a human’s life, the cardiovascular system is modulated by factors both intrinsic and extrinsic to the physiology of the heart. We can uncover new insights regarding the nature of our system through investigations of similar systems in other model species. One example materializes itself in the form of the American Lobster (Homarus americanus) whose single-chambered heart finds resemblance to the function and anatomy to that of humans. The lobster heart is powered by the cardiac ganglion (CG), a group of neurons that drive contractions of surrounding heart muscles, known as the myocardium. Both the CG and myocardium work in a feedback loop, with both intrinsic (afterload and preload) and extrinsic (temperature and neuropeptides) factors affecting cardiac output (CO) or the overall ability of the heart to carry out its primary function of nutrient distribution. In this paper, we examine how the addition of these factors into in vitro whole heart preparations affect CO and other associated variables. From experimentation, we conclude that the neuropeptide SGRNFLRFamide (SGRN) increases the heartbeat frequency and the active force exerted by the heart. We also conclude that increases in temperature decrease CO as higher temperatures decrease heartbeat frequency and the active force exerted by the heart. Lastly, we conclude that the effect of preload and afterload combined produce more robust effects on the CO and active force of the heart, potentially painting a better picture of what may happen in vivo.